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The possible role of hypoxia in the affected tissue of relapsed clubfoot

SYSNO ASEP	0556129
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The possible role of hypoxia in the affected tissue of relapsed clubfoot
Author(s)	Novotný, T. (CZ) Eckhardt, Adam (FGU-C)_{RID, ORCID} Doubková, Martina (FGU-C)_{ORCID, RID} Knitlová, Jarmila (FGU-C)_{RID, ORCID} Vondrášek, David (FGU-C)_{ORCID, SAI} Vaňásková, E. (CZ) Ošťádal, M. (CZ) Uhlík, J. (CZ) Bačáková, Lucie (FGU-C)_{RID, ORCID} Musílková, Jana (FGU-C)_{RID, ORCID}
Number of authors	10
Article number	4462
Source Title	Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322 Roč. 12, č. 1 (2022)
Number of pages	10 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	matrix metalloproteinases ; ponseti method ; hemoglobin ; expression ; beta ; growth ; alpha ; proliferation ; angiogenesis ; hif-1-alpha
OECD category	Orthopaedics
R&D Projects	NV17-31564A GA MZd - Ministry of Health (MZ)
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823
UT WOS	000769466200014
EID SCOPUS	85126640231
DOI	10.1038/s41598-022-08519-z
Annotation	Our aim was to study the expression of hypoxia-related proteins as a possible regulatory pathway in the contracted side tissue of relapsed clubfoot. We compared the expression of hypoxia-related proteins in the tissue of the contracted (medial) side of relapsed clubfoot, and in the tissue of the non-contracted (lateral) side of relapsed clubfoot. Tissue samples from ten patients were analyzed by immunohistochemistry and image analysis, Real-time PCR and Mass Spectrometry to evaluate the differences in protein composition and gene expression. We found a significant increase in the levels of smooth muscle actin, transforming growth factor-beta, hypoxia-inducible factor 1 alpha, lysyl oxidase, lysyl oxidase-like 2, tenascin C, matrix metalloproteinase-2, matrix metalloproteinase-9, fibronectin, collagen types III and VI, hemoglobin subunit alpha and hemoglobin subunit beta, and an overexpression of ACTA2, FN1, TGFB1, HIF1A and MMP2 genes in the contracted medial side tissue of clubfoot. In the affected tissue, we have identified an increase in the level of hypoxia-related proteins, together with an overexpression of corresponding genes. Our results suggest that the hypoxia-associated pathway is potentially a factor contributing to the etiology of clubfoot relapses, as it stimulates both angioproliferation and fibroproliferation, which are considered to be key factors in the progression and development of relapses.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2023
Electronic address	https://doi.org/10.1038/s41598-022-08519-z

Number of the records: 1