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Unraveling the phospholipid identity of the gene expression compartments by single molecule localization microscopy

SYSNO ASEP	0555844
Document Type	O - Others
R&D Document Type	Others
Title	Unraveling the phospholipid identity of the gene expression compartments by single molecule localization microscopy
Author(s)	Hoboth, Peter (UMG-J)_ORCID Sztacho, Martin (UMG-J)_ORCID Šebesta, O. (CZ) Hozák, Pavel (UMG-J)_{RID, ORCID}
Year of issue	2021
Language	eng - English
Country	CZ - Czech Republic
Keywords	single-molecule localization microscopy ; nuclear phosphatidylinositol phosphates ; nucleoplasm ; nuclear speckles ; RNA polymerase II transcription
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Cell biology
R&D Projects	GA19-05608S GA ČR - Czech Science Foundation (CSF)
	GA18-19714S GA ČR - Czech Science Foundation (CSF)
	LTC19048 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LTC20024 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Research Infrastructure	Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i.
Institutional support	UMG-J - RVO:68378050
Annotation	Current models of gene expression acknowledge protein clustering and formation of transcriptional condensates as a driving force of gene expression. These models are mostly based on single-molecule localization microscopy (SMLM) which provided an unprecedented insight into the sub-nuclear organization of proteins and nucleic acids. However, the roles of nuclear lipids in the establishment of the functional nuclear architecture, apart from the nuclear envelope, has been neglected. Nevertheless, accumulating evidence suggests the involvement of nuclear lipids and particularly of phosphatidylinositol phosphates (PIPs) in gene expression. We used quantitative SMLM for the evaluation of the nuclear PIP distribution while preserving the context of nuclear architecture. We showed phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), phosphatidylinositol 3,4-bisphosphate (PI(3,4)P2) and phosphatidylinositol 4-monophosphate (PI(4)P) within nuclear speckles and in the nucleoplasmic foci. Moreover, we found PI(4,5)P2 and PI(3,4)P2 in the close proximity with the subset of RNA polymerase II (RNAPII) foci either in the nucleoplasm or nuclear speckles. We continue to uncover the PIP fingerprints in the subsequent stages of RNAPII transcription. Our efforts aim at elucidating the roles of nuclear PIPs in the compartmentalization of gene expression.
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2022

Number of the records: 1