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Cap analysis of gene expression reveals alternative promoter usage in a rat model of hypertension
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SYSNO ASEP 0555843 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cap analysis of gene expression reveals alternative promoter usage in a rat model of hypertension Author(s) Dahale, S. (GB)
Ruiz-Orera, J. (DE)
Šilhavý, Jan (FGU-C) RID, ORCID
Hübner, N. (DE)
van Heesch, S. (NL)
Pravenec, Michal (FGU-C) RID, ORCID
Atanur, S. S. (GB)Article number e202101234 Source Title Life Science Alliance. - : Life Science Alliance
Roč. 5, č. 4 (2022)Number of pages 13 s. Language eng - English Country US - United States Keywords cap analysis of gene expression (CAGE) ; alternative promoter usage ; spontaneously hypertensive rat ; left ventricle OECD category Biochemistry and molecular biology Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 000742638600001 EID SCOPUS 85123269301 DOI 10.26508/lsa.202101234 Annotation The role of alternative promoter usage in tissue-specific gene expression has been well established, however, its role in complex diseases is poorly understood. We performed cap analysis of gene expression (CAGE) sequencing from the left ventricle of a rat model of hypertension, the spontaneously hypertensive rat (SHR), and a normotensive strain, Brown Norway to understand the role of alternative promoter usage in complex disease. We identified 26,560 CAGE-defined transcription start sites in the rat left ventricle, including 1,970 novel cardiac transcription start sites. We identified 28 genes with alternative promoter usage between SHR and Brown Norway, which could lead to protein isoforms differing at the amino terminus between two strains and 475 promoter switching events altering the length of the 5′ UTR. We found that the shift in Insr promoter usage was significantly associated with insulin levels and blood pressure within a panel of HXB/BXH recombinant inbred rat strains, suggesting that hyperinsulinemia due to insulin resistance might lead to hypertension in SHR. Our study provides a preliminary evidence of alternative promoter usage in complex diseases. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2023 Electronic address https://doi.org/10.26508/lsa.202101234
Number of the records: 1