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Time-Dependent Changes in Protein Composition of Medial Prefrontal Cortex in Rats with Neuropathic Pain
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SYSNO ASEP 0555833 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Time-Dependent Changes in Protein Composition of Medial Prefrontal Cortex in Rats with Neuropathic Pain Author(s) Ujčíková, Hana (FGU-C) RID, ORCID
Robles, D. (US)
Yue, X. (US)
Svoboda, Petr (FGU-C) RID, ORCID
Lee, Y. S. (US)
Navratilova, E. (US)Article number 955 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 23, č. 2 (2022)Number of pages 17 s. Language eng - English Country CH - Switzerland Keywords neuropathic pain ; affective dimension of pain ; pain chronification ; prefrontal cortex ; proteomics OECD category Physiology (including cytology) Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 000747704200001 EID SCOPUS 85122780481 DOI 10.3390/ijms23020955 Annotation Chronic pain is associated with time-dependent structural and functional reorganization of the prefrontal cortex that may reflect adaptive pain compensatory and/or maladaptive pain-promoting mechanisms. However, the molecular underpinnings of these changes and whether there are time-dependent relationships to pain progression are not well characterized. In this study, we analyzed protein composition in the medial prefrontal cortex (mPFC) of rats at two timepoints after spinal nerve ligation (SNL) using two-dimensional gel electrophoresis (2D-ELFO) and liquid chromatography with tandem mass spectrometry (LC-MS/MS). SNL, but not sham-operated, rats developed persistent tactile allodynia and thermal hyperalgesia, confirming the presence of experimental neuropathic pain. Two weeks after SNL (early timepoint), we identified 11 proteins involved in signal transduction, protein transport, cell homeostasis, metabolism, and apoptosis, as well as heat-shock proteins and chaperones that were upregulated by more than 1.5-fold compared to the sham-operated rats. Interestingly, there were only four significantly altered proteins identified at 8 weeks after SNL (late timepoint). These findings demonstrate extensive time-dependent modifications of protein expression in the rat mPFC under a chronic neuropathic pain state that might underlie the evolution of chronic pain characterized by early pain-compensatory and later aberrant mechanisms. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2023 Electronic address https://www.mdpi.com/1422-0067/23/2/955
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