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Exosomes produced by melanoma cells significantly influence the biological properties of normal and cancer-associated fibroblasts
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SYSNO ASEP 0555562 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Exosomes produced by melanoma cells significantly influence the biological properties of normal and cancer-associated fibroblasts Author(s) Strnadová, K. (CZ)
Pfeiferová, Lucie (UMG-J)
Přikryl, P. (CZ)
Dvořánková, B. (CZ)
Vlčák, Erik (UMG-J)
Frýdlová, J. (CZ)
Vokurka, M. (CZ)
Novotný, Jiří (UMG-J) ORCID
Šáchová, Jana (UMG-J)
Hradilová, Miluše (UMG-J)
Brábek, J. (CZ)
Šmigová, J. (CZ)
Rösel, D. (CZ)
Smetana, K. Jr. (CZ)
Kolář, Michal (UMG-J) RID, ORCID
Lacina, L. (CZ)Number of authors 16 Source Title Histochemistry and Cell Biology. - : Springer - ISSN 0948-6143
Roč. 157, č. 2 (2022), s. 153-172Number of pages 20 s. Publication form Online - E Language eng - English Country DE - Germany Keywords Melanoma ; Cancer-associated fibroblasts ; Exosomes ; il-6 ; il-8 ; Proinflammatory cytokine Subject RIV EB - Genetics ; Molecular Biology OECD category Cell biology R&D Projects EF16_019/0000785 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF18_046/0016045 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA19-05048S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support UMG-J - RVO:68378050 UT WOS 000722977300001 DOI 10.1007/s00418-021-02052-2 Annotation The incidence of cutaneous malignant melanoma is increasing worldwide. While the treatment of initial stages of the disease is simple, the advanced disease frequently remains fatal despite novel therapeutic options . This requires identification of novel therapeutic targets in melanoma. Similarly to other types of tumours, the cancer microenvironment plays a prominent role and determines the biological properties of melanoma. Importantly, melanoma cell-produced exosomes represent an important tool of intercellular communication within this cancer ecosystem. We have focused on potential differences in the activity of exosomes produced by melanoma cells towards melanoma-associated fibroblasts and normal dermal fibroblasts. Cancer-associated fibroblasts were activated by the melanoma cell-produced exosomes significantly more than their normal counterparts, as assessed by increased transcription of genes for inflammation-supporting cytokines and chemokines, namely IL-6 or IL-8. We have observed that the response is dependent on the duration of the stimulus via exosomes and also on the quantity of exosomes. Our study demonstrates that melanoma-produced exosomes significantly stimulate the tumour-promoting proinflammatory activity of cancer-associated fibroblasts. This may represent a potential new target of oncologic therapy . Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2023 Electronic address https://link.springer.com/article/10.1007/s00418-021-02052-2
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