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Analyses of viral genomes for G-quadruplex forming sequences reveal their correlation with the type of infection
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SYSNO ASEP 0554894 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Analyses of viral genomes for G-quadruplex forming sequences reveal their correlation with the type of infection Author(s) Bohalova, Natalia (BFU-R) ORCID
Cantara, Alessio (BFU-R)
Bartas, M. (CZ)
Kaura, P. (CZ)
Šťastný, J. (CZ)
Pečinka, P. (CZ)
Fojta, Miroslav (BFU-R) RID, ORCID
Mergny, Jean-Louis (BFU-R) ORCID, RID
Brázda, Václav (BFU-R) RID, ORCIDNumber of authors 9 Source Title Biochimie. - : Elsevier - ISSN 0300-9084
Roč. 186, JUL 2021 (2021), s. 13-27Number of pages 15 s. Publication form Print - P Language eng - English Country FR - France Keywords rna ; replication ; promoter ; viruses ; binding ; zika ; G-quadruplex OECD category Biochemistry and molecular biology R&D Projects GA18-15548S GA ČR - Czech Science Foundation (CSF) EF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BFU-R - RVO:68081707 UT WOS 000662667800002 EID SCOPUS 85104058471 DOI 10.1016/j.biochi.2021.03.017 Annotation G-quadruplexes contribute to the regulation of key molecular processes. Their utilization for antiviral therapy is an emerging field of contemporary research. Here we present comprehensive analyses of the presence and localization of putative G-quadruplex forming sequences (PQS) in all viral genomes currently available in the NCBI database (including subviral agents). The G4Hunter algorithm was applied to a pool of 11,000 accessible viral genomes representing 350 Mbp in total. PQS frequencies differ across evolutionary groups of viruses, and are enriched in repeats, replication origins, 50UTRs and 30UTRs. Importantly, PQS presence and localization is connected to viral lifecycles and corresponds to the type of viral infection rather than to nucleic acid type, while viruses routinely causing persistent infections in Metazoa hosts are enriched for PQS, viruses causing acute infections are significantly depleted for PQS. The unique localization of PQS identifies the importance of G-quadruplex-based regulation of viral replication and life cycle, providing a tool for potential therapeutic targeting. (C) 2021 The Authors. Published by Elsevier B.V. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2022 Electronic address https://www.sciencedirect.com/science/article/pii/S0300908421000961?via%3Dihub
Number of the records: 1