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Changing Temperature Conditions during Somatic Embryo Maturation Result in Pinus pinaster Plants with Altered Response to Heat Stress

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    SYSNO ASEP0554678
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleChanging Temperature Conditions during Somatic Embryo Maturation Result in Pinus pinaster Plants with Altered Response to Heat Stress
    Author(s) Sales, E. (ES)
    Canizares, E. (ES)
    Pereira, C. (ES)
    Pérez-Oliver, M. A. (ES)
    Nebauer, S. (ES)
    Pavlović, Iva (UEB-Q) ORCID
    Novák, Ondřej (UEB-Q) RID, ORCID, SAI
    Segura, J. (ES)
    Arrillaga, I. (ES)
    Number of authors9
    Article number1318
    Source TitleInternational Journal of Molecular Sciences. - : MDPI
    Roč. 23, č. 3 (2022)
    Number of pages16 s.
    Languageeng - English
    CountryCH - Switzerland
    Keywordstolerance ; anatomy ; thermotolerance ; embryogenesis ; variability ; physiology ; expression ; abies ; somatic embryogenesis ; abiotic stress ; resilience ; photosynthesis ; maritime pine ; leaf anatomy
    OECD categoryPlant sciences, botany
    R&D ProjectsEF16_019/0000827 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    GA20-22875S GA ČR - Czech Science Foundation (CSF)
    Method of publishingOpen access
    Institutional supportUEB-Q - RVO:61389030
    UT WOS000754629600001
    EID SCOPUS85123890355
    DOI10.3390/ijms23031318
    AnnotationUnder the global warming scenario, obtaining plant material with improved tolerance to abiotic stresses is a challenge for afforestation programs. In this work, maritime pine (Pinus pinaster Aiton) plants were produced from somatic embryos matured at different temperatures (18, 23, or 28 degrees C, named after M18, M23, and M28, respectively) and after 2 years in the greenhouse a heat stress treatment (45 degrees C for 3 h/day for 10 days) was applied. Temperature variation during embryo development resulted in altered phenotypes (leaf histology, proline content, photosynthetic rates, and hormone profile) before and after stress. The thickness of chlorenchyma was initially larger in M28 plants, but was significantly reduced after heat stress, while increased in M18 plants. Irrespective of their origin, when these plants were subjected to a heat treatment, relative water content (RWC) and photosynthetic carbon assimilation rates were not significantly affected, although M18 plants increased net photosynthesis rate after 10 days recovery (tR). M18 plants showed proline contents that increased dramatically (2.4-fold) when subjected to heat stress, while proline contents remained unaffected in M23 and M28 plants. Heat stress significantly increased abscisic acid (ABA) content in the needles of maritime pine plants (1.4-, 3.6- and 1.9-fold in M18, M23, and M28 plants, respectively), while indole-3-acetic acid content only increased in needles from M23 plants. After the heat treatment, the total cytokinin contents of needles decreased significantly, particularly in M18 and M28 plants, although levels of active forms (cytokinin bases) did not change in M18 plants. In conclusion, our results suggest that maturation of maritime pine somatic embryos at lower temperature resulted in plants with better performance when subjected to subsequent high temperature stress, as demonstrated by faster and higher proline increase, lower increases in ABA levels, no reduction in active cytokinin, and a better net photosynthesis rate recovery.
    WorkplaceInstitute of Experimental Botany
    ContactDavid Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
    Year of Publishing2022
    Electronic addresshttp://doi.org/10.3390/ijms23031318
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