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The Effect of Mycotoxins and Silymarin on Liver Lipidome of Mice with Non-Alcoholic Fatty Liver Disease
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SYSNO ASEP 0554370 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Effect of Mycotoxins and Silymarin on Liver Lipidome of Mice with Non-Alcoholic Fatty Liver Disease Author(s) Bechyňská, K. (CZ)
Kosek, V. (CZ)
Fenclová, M. (CZ)
Muchová, L. (CZ)
Smid, V. (CZ)
Suk, J. (CZ)
Chalupský, Karel (UMG-J)
Sticová, E. (CZ)
Hůrková, K. (CZ)
Hajslova, J. (CZ)
Vítek, L. (CZ)
Stranská, M. (CZ)Number of authors 12 Article number 1723 Source Title Biomolecules. - : MDPI
Roč. 11, č. 11 (2021)Number of pages 14 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords mycotoxins ; silymarin ; lipidome ; metabolome ; mice liver ; mass spectrometry Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects LM2018126 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED2.1.00/19.0395 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UMG-J - RVO:68378050 UT WOS 000729305600001 DOI 10.3390/biom11111723 Annotation Milk thistle-based dietary supplements have become increasingly popular. The extract from milk thistle (Silybum marianum) is often used for the treatment of liver diseases because of the presence of its active component, silymarin. However, the co-occurrence of toxic mycotoxins in these preparations is quite frequent as well. The objective of this study was to investigate the changes in composition of liver lipidome and other clinical characteristics of experimental mice fed by a high-fat methionine-choline deficient diet inducing non-alcoholic fatty liver disease. The mice were exposed to (i) silymarin, (ii) mycotoxins (trichothecenes, enniatins, beauvericin, and altertoxins) and (iii) both silymarin and mycotoxins, and results were compared to the controls. The liver tissue extracts were analyzed by ultra-high performance liquid chromatography coupled with high-resolution tandem mass spectrometry. Using tools of univariate and multivariate statistical analysis, we were able to identify 48 lipid species from the classes of diacylglycerols, triacylglycerols, free fatty acids, fatty acid esters of hydroxy fatty acids and phospholipids clearly reflecting the dysregulation of lipid metabolism upon exposure to mycotoxin and/or silymarin. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2022 Electronic address https://www.mdpi.com/2218-273X/11/11/1723
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