Number of the records: 1
Cryptosporidium myocastoris n. sp. (Apicomplexa: Cryptosporidiidae), the Species Adapted to the Nutria (Myocastor coypus)
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SYSNO ASEP 0554114 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cryptosporidium myocastoris n. sp. (Apicomplexa: Cryptosporidiidae), the Species Adapted to the Nutria (Myocastor coypus) Author(s) Ježková, J. (CZ)
Limpouchová, Zlata (BC-A) SAI
Prediger, J. (CZ)
Holubová, Nikola (BC-A) SAI, ORCID
Sak, Bohumil (BC-A) RID, ORCID
Konečný, R. (CZ)
Květoňová, Dana (BC-A) SAI
Hlásková, Lenka (BC-A) SAI
Rost, M. (CZ)
McEvoy, J. (US)
Rajský, D. (SK)
Feng, Y. (CN)
Kváč, Martin (BC-A) RID, RID, ORCIDNumber of authors 13 Article number 813 Source Title Microorganisms. - : MDPI
Roč. 9, č. 4 (2021)Number of pages 24 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords molecular characterization ; discontinuous sucrose ; giardia-duodenalis ; waste-water ; life-cycle ; parvum ; prevalence ; spp. ; cattle ; parasites ; adaptation ; prevalence ; biology ; course of infection ; infectivity ; oocyst size ; phylogeny ; parasite Subject RIV GJ - Animal Vermins ; Diseases, Veterinary Medicine OECD category Veterinary science R&D Projects LTAUSA17165 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 000643299800001 EID SCOPUS 85103911182 DOI 10.3390/microorganisms9040813 Annotation Cryptosporidium spp., common parasites of vertebrates, remain poorly studied in wildlife. This study describes the novel Cryptosporidium species adapted to nutrias (Myocastor coypus). A total of 150 faecal samples of feral nutria were collected from locations in the Czech Republic and Slovakia and examined for Cryptosporidium spp. oocysts and specific DNA at the SSU, actin, HSP70, and gp60 loci. Molecular analyses revealed the presence of C. parvum (n = 1), C. ubiquitum subtype family XIId (n = 5) and Cryptosporidium myocastoris n. sp. XXIIa (n = 2), and XXIIb (n = 3). Only nutrias positive for C. myocastoris shed microscopically detectable oocysts, which measured 4.8-5.2 x 4.7-5.0 mu m, and oocysts were infectious for experimentally infected nutrias with a prepatent period of 5-6 days, although not for mice, gerbils, or chickens. The infection was localised in jejunum and ileum without observable macroscopic changes. The microvilli adjacent to attached stages responded by elongating. Clinical signs were not observed in naturally or experimentally infected nutrias. Phylogenetic analyses at SSU, actin, and HSP70 loci demonstrated that C. myocastoris n. sp. is distinct from other valid Cryptosporidium species. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2022 Electronic address https://www.mdpi.com/2076-2607/9/4/813
Number of the records: 1