Number of the records: 1

Size and Fluorescence Properties of Algal Photosynthetic Antenna Proteins Estimated by Microscopy

SYSNO ASEP	0553484
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Size and Fluorescence Properties of Algal Photosynthetic Antenna Proteins Estimated by Microscopy
Author(s)	Crepin, Aurelia (MBU-M)_ORCID Belgio, Erica (MBU-M) Šedivá, Barbora (MBU-M) Trsková, Eliška (MBU-M) Cunill-Semanat, Edel (MBU-M) Kaňa, Radek (MBU-M)_{RID, ORCID}
Article number	778
Source Title	International Journal of Molecular Sciences. - : MDPI Roč. 23, č. 2 (2022)
Number of pages	10 s.
Language	eng - English
Country	CH - Switzerland
Keywords	light-harvesting-complex ; photosystem-ii ; chromera-velia ; membrane ; supercomplex ; acclimation ; plants ; photosynthesis ; antenna proteins ; light-harvesting ; fluorescence correlation spectroscopy ; protein diffusion ; microscopy ; protein oligomerization ; Chromera velia
Subject RIV	CE - Biochemistry
OECD category	Biochemistry and molecular biology
R&D Projects	GA19-11494S GA ČR - Czech Science Foundation (CSF)
	LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED2.1.00/19.0392 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	MBU-M - RVO:61388971
UT WOS	000746412300001
EID SCOPUS	85122491782
DOI	10.3390/ijms23020778
Annotation	Antenna proteins play a major role in the regulation of light-harvesting in photosynthesis. However, less is known about a possible link between their sizes (oligomerization state) and fluorescence intensity (number of photons emitted). Here, we used a microscopy-based method, Fluorescence Correlation Spectroscopy (FCS), to analyze different antenna proteins at the particle level. The direct comparison indicated that Chromera Light Harvesting (CLH) antenna particles (isolated from Chromera velia) behaved as the monomeric Light Harvesting Complex II (LHCII) (from higher plants), in terms of their radius (based on the diffusion time) and fluorescence yields. FCS data thus indicated a monomeric oligomerization state of algal CLH antenna (at our experimental conditions) that was later confirmed also by biochemical experiments. Additionally, our data provide a proof of concept that the FCS method is well suited to measure proteins sizes (oligomerization state) and fluorescence intensities (photon counts) of antenna proteins per single particle (monomers and oligomers). We proved that antenna monomers (CLH and LHCIIm) are more ´quenched´ than the corresponding trimers. The FCS measurement thus represents a useful experimental approach that allows studying the role of antenna oligomerization in the mechanism of photoprotection.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2023
Electronic address	https://www.mdpi.com/1422-0067/23/2/778

Number of the records: 1