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Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana
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SYSNO ASEP 0552833 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana Author(s) Kashkan, Ivan (UEB-Q) ORCID
Hrtyan, M. (CZ)
Retzer, Katarzyna (UEB-Q) ORCID
Humpolíčková, Jana (UOCHB-X) ORCID
Jayasree, A. (CZ)
Filepová, Roberta (UEB-Q) SAI
Vondráková, Zuzana (UEB-Q) RID, ORCID
Simon, Sibu (UEB-Q) ORCID
Rombaut, D. (BE)
Jacobs, T. B. (BE)
Frilander, M. J. (AT)
Hejátko, J. (CZ)
Friml, J. (AT)
Petrášek, Jan (UEB-Q) RID, ORCID
Růžička, Kamil (UEB-Q) ORCIDNumber of authors 15 Source Title New Phytologist - ISSN 0028-646X
Roč. 233, č. 1 (2022), s. 329-343Number of pages 15 s. Language eng - English Country GB - United Kingdom Keywords alternative splicing ; Arabidopsis thaliana ; auxin ; auxin transport ; frap ; PINs ; plant development ; RNA processing OECD category Developmental biology R&D Projects GA19-23773S GA ČR - Czech Science Foundation (CSF) GA19-18917S GA ČR - Czech Science Foundation (CSF) EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 ; UOCHB-X - RVO:61388963 UT WOS 000714678100001 EID SCOPUS 85118492063 DOI 10.1111/nph.17792 Annotation Advanced transcriptome sequencing has revealed that the majority of eukaryotic genes undergo alternative splicing (AS). Nonetheless, little effort has been dedicated to investigating the functional relevance of particular splicing events, even those in the key developmental and hormonal regulators. Combining approaches of genetics, biochemistry and advanced confocal microscopy, we describe the impact of alternative splicing on the PIN7 gene in the model plant Arabidopsis thaliana. PIN7 encodes a polarly localized transporter for the phytohormone auxin and produces two evolutionarily conserved transcripts, PIN7a and PIN7b. PIN7a and PIN7b, differing in a four amino acid stretch, exhibit almost identical expression patterns and subcellular localization. We reveal that they are closely associated and mutually influence each other's mobility within the plasma membrane. Phenotypic complementation tests indicate that the functional contribution of PIN7b per se is minor, but it markedly reduces the prominent PIN7a activity, which is required for correct seedling apical hook formation and auxin-mediated tropic responses. Our results establish alternative splicing of the PIN family as a conserved, functionally relevant mechanism, revealing an additional regulatory level of auxin-mediated plant development. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2022 Electronic address http://doi.org/10.1111/nph.17792
Number of the records: 1