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Analysis of bacteriophage-host interaction by Raman tweezers

SYSNO ASEP	0552214
Document Type	A - Abstract
R&D Document Type	The record was not marked in the RIV
R&D Document Type	Není vybrán druh dokumentu
Title	Analysis of bacteriophage-host interaction by Raman tweezers
Author(s)	Pilát, Zdeněk (UPT-D)_{RID, SAI, ORCID} Jonáš, Alexandr (UPT-D)_{RID, SAI, ORCID} Pilátová, J. (CZ) Klementová, Tereza (UPT-D)_{ORCID, SAI} Bernatová, Silvie (UPT-D)_{RID, SAI} Šiler, Martin (UPT-D)_{RID, ORCID, SAI} Maňka, Tadeáš (UPT-D)_{RID, ORCID, SAI} Kizovský, Martin (UPT-D) Růžička, F. (CZ) Pantůček, R. (CZ) Neugebauer, U. (DE) Samek, Ota (UPT-D)_{RID, ORCID, SAI} Zemánek, Pavel (UPT-D)_{RID, SAI, ORCID}
Source Title	LA61. Sborník příspěvků multioborové konference LASER61. - Brno : Ústav přístrojové techniky AV ČR, 2021 / Růžička B. - ISBN 978-80-87441-28-2 S. 67
Number of pages	1 s.
Publication form	Print - P
Action	LASER61
Event date	20.10.2021 - 22.10.2021
VEvent location	Lednice
Country	CZ - Czech Republic
Event type	CST
Language	eng - English
Country	CZ - Czech Republic
Institutional support	UPT-D - RVO:68081731
Annotation	Bacteriophages, or “phages” for short, are viruses that replicate in bacteria. The therapeutic and biotechnological potential of phages and their lytic enzymes is based on their ability to selectively destroy pathogenic bacteria, including antibiotic-resistant strains. Introduction of phage preparations into medicine, biotechnology, and food industry requires a thorough characterization of phage-host interaction on a molecular level. We employed Raman tweezers to analyze phage-host interaction of Staphylococcus aureus strain FS159 with a virulent phage JK2 (812K1/420) of the Myoviridae family and a temperate phage 80α of the Siphoviridae family. We analyzed the timeline of phage-induced molecular changes in infected host cells. We reliably detected the presence of phages in bacterial cells within five minutes after infection. Our results lay the foundations for building a Raman-based diagnostic instrument capable of real-time, in vivo, in situ, nondestructive characterization of the phage-host relationship on the level of single cells, which has the potential of importantly contributing to the development of phage therapy and enzybiotics.
Workplace	Institute of Scientific Instruments
Contact	Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
Year of Publishing	2022

Number of the records: 1