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Analysis of bacteriophage-host interaction by Raman tweezers
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SYSNO ASEP 0552214 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Analysis of bacteriophage-host interaction by Raman tweezers Author(s) Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Jonáš, Alexandr (UPT-D) RID, SAI, ORCID
Pilátová, J. (CZ)
Klementová, Tereza (UPT-D) ORCID, SAI
Bernatová, Silvie (UPT-D) RID, SAI
Šiler, Martin (UPT-D) RID, ORCID, SAI
Maňka, Tadeáš (UPT-D) RID, ORCID, SAI
Kizovský, Martin (UPT-D)
Růžička, F. (CZ)
Pantůček, R. (CZ)
Neugebauer, U. (DE)
Samek, Ota (UPT-D) RID, ORCID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCIDSource Title LA61. Sborník příspěvků multioborové konference LASER61. - Brno : Ústav přístrojové techniky AV ČR, 2021 / Růžička B. - ISBN 978-80-87441-28-2
S. 67Number of pages 1 s. Publication form Print - P Action LASER61 Event date 20.10.2021 - 22.10.2021 VEvent location Lednice Country CZ - Czech Republic Event type CST Language eng - English Country CZ - Czech Republic Institutional support UPT-D - RVO:68081731 Annotation Bacteriophages, or “phages” for short, are viruses that replicate in bacteria. The therapeutic and biotechnological potential of phages and their lytic enzymes is based on their ability to selectively destroy pathogenic bacteria, including antibiotic-resistant strains. Introduction of phage preparations into medicine, biotechnology, and food industry requires a thorough characterization of phage-host interaction on a molecular level. We employed Raman tweezers to analyze phage-host interaction of Staphylococcus aureus strain FS159 with a virulent phage JK2 (812K1/420) of the Myoviridae family and a temperate phage 80α of the Siphoviridae family. We analyzed the timeline of phage-induced molecular changes in infected host cells. We reliably detected the presence of phages in bacterial cells within five minutes after infection. Our results lay the foundations for building a Raman-based diagnostic instrument capable of real-time, in vivo, in situ, nondestructive characterization of the phage-host relationship on the level of single cells, which has the potential of importantly contributing to the development of phage therapy and enzybiotics. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2022
Number of the records: 1