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Age-Dependent Changes in the Function of Mitochondrial Membrane Permeability Transition Pore in Rat Liver Mitochondria
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SYSNO ASEP 0552140 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Age-Dependent Changes in the Function of Mitochondrial Membrane Permeability Transition Pore in Rat Liver Mitochondria Author(s) Endlicher, R. (CZ)
Drahota, Zdeněk (FGU-C) RID, ORCID
Kučera, O. (CZ)
Červinková, Z. (CZ)Source Title Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 70, č. 6 (2021), s. 905-911Number of pages 7 s. Language eng - English Country CZ - Czech Republic Keywords rat liver mitochondria ; mitochondrial permeability transition pore ; calcium retention capacity ; calcium-induced swelling OECD category Physiology (including cytology) Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 000735395600009 EID SCOPUS 85122449375 DOI 10.33549/physiolres.934734 Annotation Mitochondria play an important role in the cell aging process. Changes in calcium homeostasis and/or increased reactive oxygen species (ROS) production lead to the opening of mitochondrial permeability transition pore (MPTP), depolarization of the inner mitochondrial membrane, and decrease of ATP production. Our work aimed to monitor age-related changes in the Ca2+ ion effect on MPTP and the ability of isolated rat liver mitochondria to accumulate calcium. The mitochondrial calcium retention capacity (CRC) was found to be significantly affected by the age of rats. Measurement of CRC values of the rat liver mitochondria showed two periods when 3 to 17-week old rats were tested. 3-week and 17-week old rats showed lower CRC values than 7-week old animals. Similar changes were observed while testing calcium-induced swelling of rat liver mitochondria. These findings indicate that the mitochondrial energy production system is more resistant to calcium-induced MPTP opening accompanied by the damaging effect of ROS in adult rats than in young and aged animals. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2022 Electronic address https://www.biomed.cas.cz/physiolres/pdf/2021/70_905.pdf
Number of the records: 1