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Defects in meiotic chromosome segregation lead to unreduced male gametes in Arabidopsis SMC5/6 complex mutants
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SYSNO ASEP 0552129 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Defects in meiotic chromosome segregation lead to unreduced male gametes in Arabidopsis SMC5/6 complex mutants Author(s) Yang, Fen (UEB-Q) ORCID
Fernández-Jiménez, N. (ES)
Tučková, Martina (UEB-Q) ORCID
Vrána, Jan (UEB-Q) RID, ORCID
Cápal, Petr (UEB-Q) RID, ORCID
Díaz, M. (ES)
Pradillo, M. (ES)
Pečinka, Aleš (UEB-Q) ORCID, RIDNumber of authors 8 Source Title Plant Cell. - : Oxford University Press - ISSN 1040-4651
Roč. 33, č. 9 (2021), s. 3104-3119Number of pages 16 s. Language eng - English Country US - United States Keywords DNA-DAMAGE ; HOMOLOGOUS RECOMBINATION ; PROTEIN OECD category Cell biology R&D Projects GA19-13848S GA ČR - Czech Science Foundation (CSF) LTC18026 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000702165300018 EID SCOPUS 85116038110 DOI 10.1093/plcell/koab178 Annotation Structural maintenance of chromosome 5/6 (SMC5/6) complex is a crucial factor for preserving genome stability. Here, we show that mutants for several Arabidopsis (Arabidopsis thaliana) SMC5/6 complex subunits produce triploid offspring. This phenotype is caused by a meiotic defect leading to the production of unreduced male gametes. The SMC5/6 complex mutants show an absence of chromosome segregation during the first and/or the second meiotic division, as well as a partially disorganized microtubule network. Importantly, although the SMC5/6 complex is partly required for the repair of SPO11-induced DNA double-strand breaks, the nonreduction described here is SPO11-independent. The measured high rate of ovule abortion suggests that, if produced, such defects are maternally lethal. Upon fertilization with an unreduced pollen, the unbalanced maternal and paternal genome dosage in the endosperm most likely causes seed abortion observed in several SMC5/6 complex mutants. In conclusion, we describe the function of the SMC5/6 complex in the maintenance of gametophytic ploidy in Arabidopsis. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2022 Electronic address http://doi.org/10.1093/plcell/koab178
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