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Proteome changes of plasma-derived extracellular vesicles in patients with myelodysplastic syndrome
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SYSNO ASEP 0552077 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Proteome changes of plasma-derived extracellular vesicles in patients with myelodysplastic syndrome Author(s) Pečánková, K. (CZ)
Pecherková, P. (CZ)
Gasova, Z. (CZ)
Sovová, Ž. (CZ)
Riedel, Tomáš (UMCH-V) RID, ORCID
Jäger, Eliezer (UMCH-V) ORCID, RID
Cermak, J. (CZ)
Majek, P. (CZ)Article number e0262484 Source Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 17, č. 1 (2022)Number of pages 14 s. Language eng - English Country US - United States Keywords chronic lymphocytic-leukemia ; world-health-organization ; oxidative stress Subject RIV BO - Biophysics OECD category Biophysics R&D Projects GA20-10845S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support UMCH-V - RVO:61389013 UT WOS 000741060700010 EID SCOPUS 85122617681 DOI 10.1371/journal.pone.0262484 Annotation Extracellular vesicles are released into body fluids from the majority of, if not all, cell types. Because their secretion and specific cargo (e.g., proteins) varies according to pathology, extracellular vesicles may prove a rich source of biomarkers. However, their biological and pathophysiological functions are poorly understood in hematological malignancies.Here, we investigated proteome changes in the exosome-rich fraction of the plasma of myelodysplastic syndrome patients and healthy donors. Exosome-rich fraction of the plasma was isolated using ExoQuick™: proteomes were compared and statistically processed, proteins were identified by nanoLC-MS/MS and verified using the ExoCarta and QuickGO databases. Mann-Whitney and Spearman analyses were used to statistically analyze the data. 2D western blot was used to monitor clusterin proteoforms. Statistical analyses of the data highlighted clusterin alterations as the most significant. 2D western blot showed that the clusterin changes were caused by posttranslational modifications. Moreover, there was a notable increase in the clusterin proteoform in the exosome-rich fraction of plasma of patients with more severe myelodysplastic syndrome, this corresponded with a simultaneous decrease in their plasma. This specific clusterin proteoform seems to be a promising biomarker for myelodysplastic syndrome progression. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2023 Electronic address https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0262484
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