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Individual copy number variation and extensive diversity between major MHC-DAB1 allelic lineages in the European bitterling
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SYSNO ASEP 0551782 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Individual copy number variation and extensive diversity between major MHC-DAB1 allelic lineages in the European bitterling Author(s) Talarico, L. (IT)
Bryjová, Anna (UBO-W) SAI, ORCID
Čížková, Dagmar (UBO-W) RID, SAI, ORCID
Douda, K. (CZ)
Reichard, Martin (UBO-W) RID, ORCID, SAI, SAINumber of authors 5 Source Title Immunogenetics. - : Springer - ISSN 0093-7711
Roč. 74, č. 5 (2022), s. 497-505Number of pages 9 s. Language eng - English Country DE - Germany Keywords Rhodeus amarus ; MHC class IIB ; Positive selection ; Gene duplication ; MHC supertypes ; Positively selected sites Subject RIV EG - Zoology OECD category Marine biology, freshwater biology, limnology R&D Projects GA19-05510S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support UBO-W - RVO:68081766 UT WOS 000741229700001 EID SCOPUS 85122650049 DOI 10.1007/s00251-021-01251-4 Annotation Polymorphism of the major histocompatibility complex (MHC), DAB1 gene was characterized for the first time in the European bitterling (Rhodeus amarus), a freshwater fish employed in studies of host-parasite coevolution and mate choice, taking advantage of newly designed primers coupled with high-throughput amplicon sequencing. Across 221 genotyped individuals, we detected 1-4 variants per fish, with 28% individuals possessing 3-4 variants. We identified 36 DAB1 variants, and they showed high sequence diversity mostly located within predicted antigen-binding sites, and both global and codon-specific excess of non-synonymous mutations. Despite deep divergence between two major allelic lineages, functional diversity was surprisingly low (3 supertypes). Overall, these findings suggest the role of positive and balancing selection in promotion and long-time maintenance of DAB1 polymorphism. Further investigations will clarify the role of pathogen-mediated selection to drive the evolution of DAB1 variation. Workplace Institute of Vertebrate Biology Contact Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Year of Publishing 2023 Electronic address https://link.springer.com/article/10.1007%2Fs00251-021-01251-4
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