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Effect of round-shaped silver nanoparticles on the genetic and functional diversity of soil microbial community in soil and “soil-plant” systems
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SYSNO ASEP 0551741 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Effect of round-shaped silver nanoparticles on the genetic and functional diversity of soil microbial community in soil and “soil-plant” systems Author(s) Macůrková, A. (CZ)
Maryška, Lukáš (UEB-Q) ORCID
Jindřichová, Barbora (UEB-Q) RID, ORCID
Drobníková, T. (CZ)
Vrchotová, B. (CZ)
Pospíchalová, Romana (UEB-Q)
Záruba, K. (CZ)
Hubáček, Tomáš (BC-A) RID
Siegel, J. (CZ)
Burketová, Lenka (UEB-Q) RID, ORCID
Lovecká, P. (CZ)
Valentová, O. (CZ)Number of authors 1 Article number 104165 Source Title Applied Soil Ecology. - : Elsevier - ISSN 0929-1393
Roč. 168, DEC (2021)Number of pages 16 s. Language eng - English Country NL - Netherlands Keywords Enzyme activity ; Functional diversity ; Genetic diversity ; Silver nanoparticles ; Soil microbiome OECD category Biochemical research methods Subject RIV - cooperation Biology Centre (since 2006) - Other Materials R&D Projects EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA17-10907S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support UEB-Q - RVO:61389030 ; BC-A - RVO:60077344 UT WOS 000701814300011 EID SCOPUS 85111215605 DOI 10.1016/j.apsoil.2021.104165 Annotation Silver and silver nanoparticles (AgNPs) are extensively used as antimicrobial agents in various products and consequently enter the soil ecosystem, in which they accumulate and can have adverse effects on above and belowground organisms. Since the composition of the soil microbiome could have a significant impact on soil fertility, we focused, in this study, on the effect of AgNPs on soil microbial communities. We evaluated the biomass, plant growth-promoting activities, and microbial genetic (taxonomical) and functional diversity in the soil and the “soil-plant” system using chemical, microbiological, and molecular biological methods, especially qPCR, RFLP, and REP-PCR. By compiling the results of the applied assays, we were able to correlate the changes in genetic diversity with the changes in functional diversity and their impact on the composition of soil organic matter. The effect of AgNPs was predominantly dose-dependent, whereas the concentration 100 mg kgdw−1 had the highest effect on all followed parameters. From the taxonomical point of view, AgNPs had the most significant impact on the relative abundance of Bacteroidetes, which increased in a dose-dependent manner, and Firmicutes, which decreased in a dose-dependent manner, and they caused the predominance of Ascomycota among Fungi. These changes in taxonomic diversity result in a significant decrease in the functional diversity of the microbial communities. Especially for the concentration 100 mg kgdw−1, we detected a significant decrease in the ability of the microbial community to utilize simple carbon substrates, determined by Biolog EcoPlates. We detected also a significant alteration in enzyme activity. The activity of enzymes responsible for depolymerization of complex organic matter was in a dose-dependent manner increased, whereas the highest activity reach samples amended with AgNPs at 100 mg kgdw−1. All these changes result in the accumulation of simple organic matter, which could increase osmotic pressure and have severe consequences on soil fertility. In amended soils, we detected alterations of activity of plant growth-promoting bacteria, specifically in the production of indole-3-acetic acid, 1-aminocyclopropane-1-carboxylic acid deaminase, and siderophores. All these activities are related to mitigating abiotic stress. The presence of plants in experimental soil had both a mitigating and deepening effect on the toxicity of AgNPs. Both effects probably relate to the influence of AgNPs on the selection of different microbial communities in the soil and the “soil-plant” system. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2022 Electronic address http://doi.org/10.1016/j.apsoil.2021.104165
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