Number of the records: 1
Dioxygenase for auxin oxidation 1 catalyzes the oxidation of IAA amino acid conjugates
- 1.
SYSNO ASEP 0551736 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Dioxygenase for auxin oxidation 1 catalyzes the oxidation of IAA amino acid conjugates Author(s) Müller, Karel (UEB-Q) RID, ORCID
Dobrev, Petre (UEB-Q) RID, ORCID
Pěnčík, Aleš (UEB-Q) ORCID, RID, SAI
Hošek, Petr (UEB-Q) ORCID
Vondráková, Zuzana (UEB-Q) RID, ORCID
Filepová, Roberta (UEB-Q) SAI
Malínská, Kateřina (UEB-Q) RID, ORCID
Brunoni, Federica (UEB-Q) ORCID
Helusová, Lenka (UEB-Q)
Moravec, Tomáš (UEB-Q) RID, ORCID
Retzer, Katarzyna (UEB-Q) ORCID
Harant, K. (CZ)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Hoyerová, Klára (UEB-Q) RID, ORCID
Petrášek, Jan (UEB-Q) RID, ORCIDNumber of authors 4 Source Title Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 187, č. 1 (2021), s. 103-115Number of pages 13 s. Language eng - English Country US - United States Keywords GENE-EXPRESSION ; HOMEOSTASIS ; FAMILY OECD category Plant sciences, botany R&D Projects EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTC18047 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000696244400019 EID SCOPUS 85114456402 DOI 10.1093/plphys/kiab242 Annotation Together with auxin transport, auxin metabolism is a key determinant of auxin signaling output by plant cells. Enzymatic machinery involved in auxin metabolism is subject to regulation based on numerous inputs, including the concentration of auxin itself. Therefore, experiments characterizing altered auxin availability and subsequent changes in auxin metabolism could elucidate the function and regulatory role of individual elements in the auxin metabolic machinery. Here, we studied auxin metabolism in auxin-dependent tobacco BY-2 cells. We revealed that the concentration of N-(2-oxindole-3-acetyl)-L-aspartic acid (oxIAA-Asp), the most abundant auxin metabolite produced in the control culture, dramatically decreased in auxin-starved BY-2 cells. Analysis of the transcriptome and proteome in auxin-starved cells uncovered significant downregulation of all tobacco (Nicotiana tabacum) homologs of Arabidopsis (Arabidopsis thaliana) DIOXYGENASE FOR AUXIN OXIDATION 1 (DAO1), at both transcript and protein levels. Auxin metabolism profiling in BY-2 mutants carrying either siRNA-silenced or CRISPR-Cas9-mutated NtDAO1, as well as in dao1-1 Arabidopsis plants, showed not only the expected lower levels of oxIAA, but also significantly lower abundance of oxIAA-Asp. Finally, ability of DAO1 to oxidize IAA-Asp was confirmed by an enzyme assay in AtDAO1-producing bacterial culture. Our results thus represent direct evidence of DAO1 activity on IAA amino acid conjugates. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2022 Electronic address http://doi.org/10.1093/plphys/kiab242
Number of the records: 1