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Towards spruce-type photosystem II: Consequences of the loss of light-harvesting proteins LHCB3 and LHCB6 in Arabidopsis
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SYSNO ASEP 0551720 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Towards spruce-type photosystem II: Consequences of the loss of light-harvesting proteins LHCB3 and LHCB6 in Arabidopsis Author(s) Ilikova, Iva (UEB-Q) ORCID
Ilík, P. (CZ)
Opatíková, M. (CZ)
Arshad, R. (CZ)
Nosek, L. (CZ)
Karlický, Václav (UEK-B) ORCID, RID, SAI
Kučerová, Z. (CZ)
Roudnický, P. (CZ)
Pospíšil, P. (CZ)
Lazár, D. (CZ)
Bartoš, Jan (UEB-Q) RID, ORCID
Kouřil, R. (CZ)Number of authors 0 Source Title Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 187, č. 4 (2021), s. 2691-2715Number of pages 25 s. Language eng - English Country US - United States Keywords EXCITATION-ENERGY TRANSFER ; THYLAKOID MEMBRANES ; CHLOROPHYLL FLUORESCENCE OECD category Biophysics R&D Projects GA18-12178S GA ČR - Czech Science Foundation (CSF) GA21-05497S GA ČR - Czech Science Foundation (CSF) EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_019/0000797 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018127 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 ; UEK-B - RVO:86652079 UT WOS 000733403700059 EID SCOPUS 85121125021 DOI 10.1093/plphys/kiab396 Annotation The largest stable photosystem II (PSII) supercomplex in land plants (C2S2M2) consists of a core complex dimer (C2), two strongly (S2) and two moderately (M2) bound light-harvesting protein (LHCB) trimers attached to C2 via monomeric antenna proteins LHCB4-6. Recently, we have shown that LHCB3 and LHCB6, presumably essential for land plants, are missing in Norway spruce (Picea abies), which results in a unique structure of its C2S2M2 supercomplex. Here, we performed structure- function characterization of PSII supercomplexes in Arabidopsis (Arabidopsis thaliana) mutants lhcb3, lhcb6, and lhcb3 lhcb6 to examine the possibility of the formation of the spruce-type PSII supercomplex in angiosperms. Unlike in spruce, in Arabidopsis both LHCB3 and LHCB6 are necessary for stable binding of the M trimer to PSII core. The spruce-type PSII supercomplex was observed with low abundance only in the lhcb3 plants and its formation did not require the presence of LHCB4.3, the only LHCB4-type protein in spruce. Electron microscopy analysis of grana membranes revealed that the majority of PSII in lhcb6 and namely in lhcb3 lhcb6 mutants were arranged into C2S2semi-crystalline arrays, some of which appeared to structurally restrict plastoquinone diffusion. Mutants without LHCB6 were characterized by fast induction of non-photochemical quenching and, on the contrary to the previous lhcb6 study, by only transient slowdown of electron transport between PSII and PSI. We hypothesize that these functional changes, associated with the arrangement of PSII into C2S2arrays in thylakoids, may be important for the photoprotection of both PSI and PSII upon abrupt high-light exposure. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2022 Electronic address http://doi.org/10.1093/plphys/kiab396
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