On the origin of reduced cytotoxicity of germanium‐doped diamond‐like carbon: Role of top surface composition and bonding

Zemek, Josef; Jiříček, Petr; Houdková, Jana; Ledinský, Martin; Jelínek, Miroslav; Kocourek, Tomáš

doi:https://dx.doi.org/10.3390/nano11030567

Number of the records: 1

On the origin of reduced cytotoxicity of germanium‐doped diamond‐like carbon: Role of top surface composition and bonding

1.

SYSNO ASEP	0549938
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	On the origin of reduced cytotoxicity of germanium‐doped diamond‐like carbon: Role of top surface composition and bonding
Author(s)	Zemek, Josef (FZU-D)_{RID, ORCID} Jiříček, Petr (FZU-D)_{RID, ORCID, SAI} Houdková, Jana (FZU-D)_{RID, ORCID} Ledinský, Martin (FZU-D)_{RID, ORCID, SAI} Jelínek, Miroslav (FZU-D)_{RID, ORCID} Kocourek, Tomáš (FZU-D)_{RID, ORCID, SAI}
Number of authors	6
Article number	567
Source Title	Nanomaterials. - : MDPI Roč. 11, č. 3 (2021)
Number of pages	10 s.
Language	eng - English
Country	CH - Switzerland
Keywords	germanium ; diamond-like carbon ; photoelectron spectroscopy ; low energy ion scattering spectroscopy ; Raman spectroscopy ; carbon capping film ; cytotoxic
Subject RIV	BO - Biophysics
OECD category	Biophysics
R&D Projects	EF16_026/0008382 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	FZU-D - RVO:68378271
UT WOS	000633950200001
EID SCOPUS	85101380351
DOI	10.3390/nano11030567
Annotation	This work attempts to understand the behaviour of Ge-induced cytotoxicity of germanium-doped hydrogen-free diamond-like carbon (DLC) films recently thoroughly studied and published by Jelinek et al. At a low doping level, the films showed no cytotoxicity, while at a higher doping level, the films were found to exhibit medium to high cytotoxicity. We demonstrate, using surface-sensitive methods-two-angle X-ray-induced core-level photoelectron spectroscopy (ARXPS) and Low Energy Ion Scattering (LEIS) spectroscopy, that at a low doping level, the layers are capped by a carbon film which impedes the contact of Ge species with tissue. For higher Ge content in the DLC films, oxidized Ge species are located at the top surface of the layers, provoking cytotoxicity. The present results indicate no threshold for Ge concentration in cell culture substrate to avoid a severe toxic reaction.
Workplace	Institute of Physics
Contact	Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579
Year of Publishing	2022
Electronic address	http://hdl.handle.net/11104/0325826

Number of the records: 1