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Self-assembly, drug encapsulation, and cellular uptake of block and gradient copolymers of 2-methyl-2-oxazine and 2-n-propyl/butyl-2-oxazoline

SYSNO ASEP	0549608
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Self-assembly, drug encapsulation, and cellular uptake of block and gradient copolymers of 2-methyl-2-oxazine and 2-n-propyl/butyl-2-oxazoline
Author(s)	Babuka, David (UMCH-V) Kolouchová, Kristýna (UMCH-V)_{ORCID, RID} Loukotová, Lenka (UMCH-V)_{RID, ORCID} Sedláček, O. (CZ) Groborz, Ondřej (UMCH-V)_{ORCID, RID} Škarková, A. (CZ) Zhigunov, Alexander (UMCH-V)_{RID, ORCID} Pavlova, Ewa (UMCH-V)_RID Hoogenboom, R. (BE) Hrubý, Martin (UMCH-V)_{RID, ORCID} Štěpánek, Petr (UMCH-V)_{RID, ORCID}
Source Title	Macromolecules. - : American Chemical Society - ISSN 0024-9297 Roč. 54, č. 23 (2021), s. 10667-10681
Number of pages	15 s.
Language	eng - English
Country	US - United States
Keywords	polyoxazoline ; gradient polymer ; block polymer
Subject RIV	CD - Macromolecular Chemistry
OECD category	Polymer science
R&D Projects	GA19-01602S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Limited access
Institutional support	UMCH-V - RVO:61389013
UT WOS	000752886100004
EID SCOPUS	85120725708
DOI	10.1021/acs.macromol.1c01794
Annotation	Self-assembled amphiphilic polymers have been extensively studied for various biomedical applications, as they show advantageous properties for diagnosis and therapy. In this work, we extensively compared amphiphilic copolymers of the hydrophilic monomer 2-methyl-2-oxazine (MeOzi) and the thermoresponsive or hydrophobic monomers 2-propyl-2-oxazoline (PrOx) or 2-butyl-2-oxazoline (BuOx) in both block and gradient monomer distributions. Such a head-to-head comparison between block and gradient copolymers, which has thus far been mostly missing in the available literature, should provide important insight into the differences and similarities between these two architectures. We investigated the properties of our polymers using a wide array of analytical methods, including dynamic light scattering (DLS), small-angle neutron (SANS) and X-ray scattering (SAXS), one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopy, transmission electron microscopy (TEM), drug loading (DL), cellular uptake, and cytotoxicity studies. Most of the studied polymers formed self-assembled nanoparticles, but their properties varied with the monomer ratio, polymer length, and polymer architecture, and these factors could be used to fine-tune the properties of the polymer to meet the demands of the desired application. Both block and gradient copolymers showed similar critical association concentrations and DL properties for the antituberculosis drug rifampicin. Finally, we confirmed that the nanoparticles could be internalized by macrophages, which indicates great potential for the utilization of these nanoparticles in drug delivery.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2022
Electronic address	https://pubs.acs.org/doi/10.1021/acs.macromol.1c01794

Number of the records: 1