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Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers

SYSNO ASEP	0549488
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers
Author(s)	Bernatová, Silvie (UPT-D)_{RID, SAI} Rebrošová, K. (CZ) Pilát, Zdeněk (UPT-D)_{RID, SAI, ORCID} Šerý, Mojmír (UPT-D)_{RID, SAI} Gjevik, Alžběta (UPT-D) Samek, Ota (UPT-D)_{RID, ORCID, SAI} Ježek, Jan (UPT-D)_{RID, ORCID, SAI} Šiler, Martin (UPT-D)_{RID, ORCID, SAI} Kizovský, Martin (UPT-D) Klementová, Tereza (UPT-D)_{ORCID, SAI} Holá, V. (CZ) Růžička, F. (CZ) Zemánek, Pavel (UPT-D)_{RID, SAI, ORCID}
Number of authors	13
Article number	233
Source Title	European Physical Journal Plus. - : Springer - ISSN 2190-5444 Roč. 136, č. 2 (2021)
Number of pages	10 s.
Publication form	Online - E
Language	eng - English
Country	DE - Germany
Keywords	Raman spectroscopy ; Staphylococcus aureus
Subject RIV	BH - Optics, Masers, Lasers
OECD category	Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect)
R&D Projects	GA19-20697S GA ČR - Czech Science Foundation (CSF)
	GF19-29651L GA ČR - Czech Science Foundation (CSF)
	EF15_003/0000476 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	UPT-D - RVO:68081731
UT WOS	000621061800003
EID SCOPUS	85101346119
DOI	10.1140/epjp/s13360-021-01152-1
Annotation	Methicillin-resistant Staphylococcus aureus is a bacterium pathogenic to humans and a leading cause of the hospital-acquired infections, causing significant increase in morbidity and mortality. Conventional antibiotic sensitivity testing requires culturing of the isolated pathogen in the presence of antibiotics, and it takes at least 48 hours. Comparatively faster determination of bacterial sensitivity to antibiotics can be achieved with Raman tweezers-an analytical method based on Raman spectroscopy and optical trapping. This article demonstrates the effectiveness of this approach for the discrimination between a methicillin-resistant and a methicillin-sensitive strain of Staphylococcus aureus in about 4 hours from a microliter volume of the bacterial sample. We found that the antibiotic-induced changes in the bacterial cells influenced the ratio of the Raman signals of nucleic acids to phenylalanine. This points to the antibiotic causing cell lysis and the associated loss of nucleic acids from the cytoplasm.
Workplace	Institute of Scientific Instruments
Contact	Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
Year of Publishing	2022
Electronic address	https://link.springer.com/article/10.1140%2Fepjp%2Fs13360-021-01152-1

Number of the records: 1