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Targeted volatolomics of human monocytes: Comparison of 2D‐GC/TOF‐MS and 1D‐GC/Orbitrap‐MS methods
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SYSNO ASEP 0549181 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Targeted volatolomics of human monocytes: Comparison of 2D‐GC/TOF‐MS and 1D‐GC/Orbitrap‐MS methods Author(s) Zemánková, K. (CZ)
Pavelicová, K. (CZ)
Pompeiano, A. (CZ)
Mravcová, L. (CZ)
Černý, M. (CZ)
Bendíčková, K. (CZ)
Hortová Kohoutková, M. (CZ)
Dryahina, Kseniya (UFCH-W) RID, ORCID
Vaculovičová, M. (CZ)
Frič, J. (CZ)
Vaníčková, L. (CZ)Article number 122975 Source Title Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. - : Elsevier - ISSN 1570-0232
Roč. 1184, NOV 2021 (2021)Number of pages 10 s. Language eng - English Country NL - Netherlands Keywords biomarker ; volatile organic compounds ; monocytes Subject RIV CF - Physical ; Theoretical Chemistry OECD category Physical chemistry Method of publishing Limited access Institutional support UFCH-W - RVO:61388955 UT WOS 000710634000005 EID SCOPUS 85118774008 DOI 10.1016/j.jchromb.2021.122975 Annotation Blood is a complex biological matrix providing valuable information on nutritional, metabolic, and immune status. The detection of blood biomarkers requires sensitive analytical methods because analytes are at very low concentrations. Peripheral blood monocytes play a crucial role in inflammatory processes, and the metabolites released by monocytes during these processes might serve as important signalling molecules and biomarkers of particular physiological states. Headspace solid-phase microextraction (HS-SPME) combined with two different mass spectrometric platforms, two-dimensional (2D) gas chromatography coupled to time-of-flight mass spectrometry (2D-GC/TOF-MS) and one-dimensional gas chromatography coupled to Orbitrap mass spectrometry (GC/Orbitrap‐MS), were applied for the investigation of volatile organic compounds (VOCs) produced by human peripheral blood monocytes. An optimized method was subsequently applied for the characterization of changes in VOCs induced by lipopolysaccharides (LPS) and zymosan (ZYM) stimulation. Overall, the 2D‐GC/TOF‐MS and the 1D‐GC/Orbitrap‐MS analyses each yielded about 4000 and 400 peaks per sample, respectively. In total, 91 VOCs belonging to eight different chemical classes were identified. The samples were collected in two fractions, conditioned media for monitoring extracellularly secreted molecules and cell pellet samples to determine the intracellular composition of VOCs. Alcohols, ketones, and hydrocarbons were the main chemical classes of the metabolic profile identified in cell fractions. Aldehydes, acids and cyclic compounds were characteristic of the conditioned media fraction. Here we demonstrate that HS-SPME-2D‐GC/TOF‐MS is more suitable for the identification of specific VOC profiles produced by human monocytes than 1D‐GC/Orbitrap‐MS. We define the signature of VOCs occurring early after monocyte activation and characterise the signalling compounds released by immune cells into media. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2022 Electronic address http://hdl.handle.net/11104/0325197
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