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Biotechnological Conversion of Grape Pomace to Poly(3-hydroxybutyrate) by Moderately Thermophilic Bacterium Tepidimonas taiwanensis
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SYSNO ASEP 0548661 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Biotechnological Conversion of Grape Pomace to Poly(3-hydroxybutyrate) by Moderately Thermophilic Bacterium Tepidimonas taiwanensis Author(s) Kouřilová, X. (CZ)
Pernicová, I. (CZ)
Vidláková, M. (CZ)
Krejčiřík, R. (CZ)
Mrázová, Kateřina (UPT-D) SAI, ORCID, RID
Hrubanová, Kamila (UPT-D) RID, SAI, ORCID
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Nebesářová, Jana (BC-A) RID, ORCID
Obruča, S. (CZ)Number of authors 9 Article number 141 Source Title Bioengineering. - : MDPI - ISSN 2306-5354
Roč. 8, č. 10 (2021)Number of pages 13 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords polyhydroxyalkanoates ; Tepidimonas taiwanensis ; grape pomace ; thermophiles Subject RIV EI - Biotechnology ; Bionics OECD category Environmental biotechnology Subject RIV - cooperation Biology Centre (since 2006) - Genetics ; Molecular Biology R&D Projects GA19-20697S GA ČR - Czech Science Foundation (CSF) LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UPT-D - RVO:68081731 ; BC-A - RVO:60077344 UT WOS 000715110900001 EID SCOPUS 85120703796 DOI 10.3390/bioengineering8100141 Annotation Polyhydroxyalkanoates (PHA) are microbial polyesters that have recently come to the forefront of interest due to their biodegradability and production from renewable sources. A potential increase in competitiveness of PHA production process comes with a combination of the use of thermophilic bacteria with the mutual use of waste substrates. In this work, the thermophilic bacterium Tepidimonas taiwanensis LMG 22826 was identified as a promising PHA producer. The ability to produce PHA in T. taiwanensis was studied both on genotype and phenotype levels. The gene encoding the Class I PHA synthase, a crucial enzyme in PHA synthesis, was detected both by genome database search and by PCR. The microbial culture of T. taiwanensis was capable of efficient utilization of glucose and fructose. When cultivated on glucose as the only carbon source at 50 °C, the PHA titers reached up to 3.55 g/L, and PHA content in cell dry mass was 65%. The preference of fructose and glucose opens the possibility to employ T. taiwanensis for PHA production on various food wastes rich in these abundant sugars. In this work, PHA production on grape pomace extracts was successfully tested. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2022 Electronic address https://www.mdpi.com/2306-5354/8/10/141
Number of the records: 1