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Upconversion nanoparticles as labels for histopathological tissue evaluation
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SYSNO ASEP 0548556 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Upconversion nanoparticles as labels for histopathological tissue evaluation Author(s) Mickert, M. J. (DE)
Farka, Z. (CZ)
Krawczyka, K. (SE)
Hlaváček, Antonín (UIACH-O) ORCID
Brandmeier, J. C. (DE)
Andersson-Engels, S.
Sjögren, A. (SE)
Skládal, P. (CZ)
Gorris, H. H. (DE)Number of authors 9 Source Title UPCONline 2021 – Conference program. -, 2021
S. 56-56Number of pages 1 s. Publication form Online - E Action Conference on Properties, Design, and Applications of Upconversion Nanomaterials /3./ Event date 06.04.2021 - 09.04.2021 VEvent location Zoom Worldwide Country FR - France Event type WRD Language eng - English Country FR - France Keywords photon-upconversion ; immunohistochemistry ; immunocytochemistry Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry Institutional support UIACH-O - RVO:68081715 Annotation In this work, we designed streptavidin-modified UCNPs (SA-UCNP) for the detection of the breast cancer biomarker HER2 on the surface of model breast cancer cell lines (Figure 1A-B) and breast cancer tissue (Figure 1C). The SA-UCNP conjugate achieved a significantly higher signal-to-background ratio compared to a conventional labelling using a streptavidin-carboxyfluorescein conjugate under the same experimental conditions [2]. An in-house made UCNP-conjugate from Lumito was used to label HER2 in breast cancer tissue. The imaging was performed by Lumito’s prototype scanner capable of combining UCNP upconversion imaging and haematoxylin brightfield imaging at a 20× magnification. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2022
Number of the records: 1