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Computational image enhancement of multimode fibre-based holographic endo-microscopy: harnessing the muddy modes
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SYSNO ASEP 0548489 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Computational image enhancement of multimode fibre-based holographic endo-microscopy: harnessing the muddy modes Author(s) Tučková, Tereza (UPT-D) ORCID
Šiler, Martin (UPT-D) RID, ORCID, SAI
Boonzajer Flaes, D. E. (DE)
Jákl, Petr (UPT-D) RID, ORCID, SAI
Turtaev, S. (DE)
Krátký, Stanislav (UPT-D) RID, ORCID, SAI
Heintzmann, R. (DE)
Uhlířová, Hana (UPT-D) ORCID, RID
Čižmár, Tomáš (UPT-D) RID, ORCID, SAINumber of authors 9 Source Title Optics Express. - : Optical Society of America - ISSN 1094-4087
Roč. 29, č. 23 (2021), s. 38206-38220Number of pages 15 s. Publication form Online - E Language eng - English Country US - United States Keywords multimode optical fibre ; image reconstruction ; wavefront shaping ; poisson noise Subject RIV BH - Optics, Masers, Lasers OECD category Optics (including laser optics and quantum optics) R&D Projects LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF15_003/0000476 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UPT-D - RVO:68081731 UT WOS 000716468800098 EID SCOPUS 85118720511 DOI 10.1364/OE.434848 Annotation In imaging geometries, which employ wavefront-shaping to control the light transport through a multi-mode optical fibre (MMF), this terminal hair-thin optical component acts as a minimally invasive objective lens, enabling high resolution laser-scanning fluorescence microscopy inside living tissues at depths hardly accessible by any other light-based technique. Even in the most advanced systems, the diffraction-limited foci scanning the object across the focal plane are contaminated by a stray optical signal carrying typically few tens of % of the total optical power. The stray illumination takes the shape of a randomised but reproducible speckle, and is unique for each position of the focus. We experimentally demonstrate that the performance of imaging a fluorescent object can be significantly improved, when resulting images are computationally post-processed, utilising records of intensities of all speckle-contaminated foci used in the imaging procedure. We present two algorithms based on a regularised iterative inversion and regularised direct pseudo-inversion respectively which lead to enhancement of the image contrast and resolution. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2022 Electronic address https://www.osapublishing.org/oe/fulltext.cfm?uri=oe-29-23-38206&id=464465
Number of the records: 1