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Epigenetic distribution of recombinant plant chromosome fragments in a human–arabidopsis hybrid cell line
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SYSNO ASEP 0547644 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Epigenetic distribution of recombinant plant chromosome fragments in a human–arabidopsis hybrid cell line Author(s) Liaw, Y. M. (JP)
Liu, Y. (AU)
Teo, C. H. (MY)
Cápal, Petr (UEB-Q) RID, ORCID
Wada, N. (JP)
Fukui, K. (JP)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Ohmido, N. (JP)Number of authors 8 Article number 5426 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 22, č. 11 (2021)Number of pages 15 s. Language eng - English Country CH - Switzerland Keywords Arabidopsis genome ; DNA methylation ; Epigenome ; Gene expression ; Human–plant hybrid cell line ; Whole-genome bisulfite sequencing (WGBS) OECD category Biochemistry and molecular biology R&D Projects EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000660187400001 EID SCOPUS 85106293642 DOI 10.3390/ijms22115426 Annotation Methylation systems have been conserved during the divergence of plants and animals, although they are regulated by different pathways and enzymes. However, studies on the interactions of the epigenomes among evolutionarily distant organisms are lacking. To address this, we studied the epigenetic modification and gene expression of plant chromosome fragments (~30 Mb) in a human–Arabidopsis hybrid cell line. The whole-genome bisulfite sequencing results demonstrated that recombinant Arabidopsis DNA could retain its plant CG methylation levels even without functional plant methyltransferases, indicating that plant DNA methylation states can be maintained even in a different genomic background. The differential methylation analysis showed that the Arabidopsis DNA was undermethylated in the centromeric region and repetitive elements. Several Arabidopsis genes were still expressed, whereas the expression patterns were not related to the gene function. We concluded that the plant DNA did not maintain the original plant epigenomic landscapes and was under the control of the human genome. This study showed how two diverging genomes can coexist and provided insights into epigenetic modifications and their impact on the regulation of gene expressions between plant and animal genomes. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2022 Electronic address http://doi.org/10.3390/ijms22115426
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