Number of the records: 1

Metabolomics atlas of oral 13C-glucose tolerance test in mice

SYSNO ASEP	0547418
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Metabolomics atlas of oral 13C-glucose tolerance test in mice
Author(s)	Lopes, Magno (FGU-C) Brejchová, Kristýna (FGU-C)_{ORCID, RID, SAI} Riečan, Martin (FGU-C)_ORCID Nováková, Michaela (FGU-C) Rossmeisl, Martin (FGU-C)_{RID, ORCID} Čajka, Tomáš (FGU-C)_{RID, ORCID, SAI} Kuda, Ondřej (FGU-C)_{RID, ORCID, SAI}
Article number	109833
Source Title	Cell Reports. - : Cell Press - ISSN 2211-1247 Roč. 37, č. 2 (2021)
Number of pages	11 s.
Language	eng - English
Country	US - United States
Keywords	metabolomics atlas ; metabolomics ; lipidomics ; glucose tolerance ; tracer analysis ; metabolite cycling ; brown adipose tissue ; 13C ; pathway analysis ; de novo lipogenesis
Subject RIV	CE - Biochemistry
OECD category	Biochemistry and molecular biology
R&D Projects	GA20-00317S GA ČR - Czech Science Foundation (CSF)
Research Infrastructure	e-INFRA CZ - 90140 - CESNET, zájmové sdružení právnických osob
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823
UT WOS	000707017600029
EID SCOPUS	85116942068
DOI	10.1016/j.celrep.2021.109833
Annotation	Glucose tolerance represents a complex phenotype in which many tissues play important roles and interact to regulate metabolic homeostasis. Here, we perform an analysis of 13C6-glucose tissue distribution, which maps the metabolome and lipidome across 12 metabolically relevant mouse organs and plasma, with integrated 13C6-glucose-derived carbon tracing during oral glucose tolerance test (OGTT). We measure time profiles of water-soluble metabolites and lipids and integrate the global metabolite response into metabolic pathways. During the OGTT, glucose use is turned on with specific kinetics at the organ level, but fasting substrates like β-hydroxybutyrate are switched off in all organs simultaneously. Timeline profiling of 13C-labeled fatty acids and triacylglycerols across tissues suggests that brown adipose tissue may contribute to the circulating fatty acid pool at maximal plasma glucose levels. The GTTAtlas interactive web application serves as a unique resource for the exploration of whole-body glucose metabolism and time profiles of tissue and plasma metabolites during the OGTT.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2022
Electronic address	https://doi.org/10.1016/j.celrep.2021.109833

Number of the records: 1