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TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley
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SYSNO ASEP 0547377 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley Author(s) Takáč, T. (CZ)
Křenek, P. (CZ)
Komis, G. (CZ)
Vadovič, P. (CZ)
Ovečka, M. (CZ)
Ohnoutková, Ludmila (UEB-Q) RID, ORCID
Pechan, T. (US)
Kašpárek, Petr (UMG-J)
Tichá, T. (CZ)
Basheer, J. (CZ)
Arick, M. (US)
Šamaj, J. (CZ)Number of authors 12 Article number 666229 Source Title Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 12, APR 29 (2021)Number of pages 23 s. Language eng - English Country CH - Switzerland Keywords barley ; chitinases ; flagellin ; HvMPK3 ; PR proteins ; proteomics ; root hairs ; talen OECD category Biochemistry and molecular biology R&D Projects EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 ; UMG-J - RVO:68378050 UT WOS 000649834600001 EID SCOPUS 85105936605 DOI 10.3389/fpls.2021.666229 Annotation Mitogen activated protein kinases (MAPKs) integrate elicitor perception with both early and late responses associated with plant defense and innate immunity. Much of the existing knowledge on the role of plant MAPKs in defense mechanisms against microbes stems from extensive research in the model plant Arabidopsis thaliana. In the present study, we investigated the involvement of barley (Hordeum vulgare) MPK3 in response to flagellin peptide flg22, a well-known bacterial elicitor. Using differential proteomic analysis we show that TALEN-induced MPK3 knock-out lines of barley (HvMPK3 KO) exhibit constitutive downregulation of defense related proteins such as PR proteins belonging to thaumatin family and chitinases. Further analyses showed that the same protein families were less prone to flg22 elicitation in HvMPK3 KO plants compared to wild types. These results were supported and validated by chitinase activity analyses and immunoblotting for HSP70. In addition, differential proteomes correlated with root hair phenotypes and suggested tolerance of HvMPK3 KO lines to flg22. In conclusion, our study points to the specific role of HvMPK3 in molecular and root hair phenotypic responses of barley to flg22. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2022 Electronic address http://doi.org/10.3389/fpls.2021.666229
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