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Production of Fucoxanthin from Phaeodactylum tricornutum Using High Performance Countercurrent Chromatography Retaining Its FOXO3 Nuclear Translocation-Inducing Effect
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SYSNO ASEP 0546834 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Production of Fucoxanthin from Phaeodactylum tricornutum Using High Performance Countercurrent Chromatography Retaining Its FOXO3 Nuclear Translocation-Inducing Effect Author(s) Bárcenas-Pérez, Daniela (MBU-M) ORCID, RID
Střížek, Antonín (MBU-M)
Hrouzek, Pavel (MBU-M) ORCID
Kopecký, Jan (MBU-M)
Barradas, M. (ES)
Sierra-Ramirez, A. (ES)
Fernandez-Marcos, P. J. (ES)
Cheel, José (MBU-M) RID, ORCIDArticle number 517 Source Title Marine Drugs. - : MDPI
Roč. 19, č. 9 (2021)Number of pages 19 s. Language eng - English Country CH - Switzerland Keywords centrifugal partition chromatography ; undaria-pinnatifida ; fucoxanthin ; Phaeodactylum tricornutum ; high performance countercurrent chromatography (HPCCC) ; countercurrent chromatography (CCC) ; centrifugal partition chromatography (CPC) Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects TN01000048 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000699484700001 EID SCOPUS 85116599987 DOI 10.3390/md19090517 Annotation Phaeodactylum tricornutum is a rich source of fucoxanthin, a carotenoid with several health benefits. In the present study, high performance countercurrent chromatography (HPCCC) was used to isolate fucoxanthin from an extract of P. tricornutum. A multiple sequential injection HPCCC method was developed combining two elution modes (reverse phase and extrusion). The lower phase of a biphasic solvent system (n-heptane, ethyl acetate, ethanol and water, ratio 5/5/6/3, v/v/v/v) was used as the mobile phase, while the upper phase was the stationary phase. Ten consecutive sample injections (240 mg of extract each) were performed leading to the separation of 38 mg fucoxanthin with purity of 97% and a recovery of 98%. The process throughput was 0.189 g/h, while the efficiency per gram of fucoxanthin was 0.003 g/h. Environmental risk and general process evaluation factors were used for assessment of the developed separation method and compared with existing fucoxanthin liquid-liquid isolation methods. The isolated fucoxanthin retained its well-described ability to induce nuclear translocation of transcription factor FOXO3. Overall, the developed isolation method may represent a useful model to produce biologically active fucoxanthin from diatom biomass. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.mdpi.com/1660-3397/19/9/517
Number of the records: 1