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Voltammetric studies of selected porphyrin G-quadruplex ligands and their interaction with DNA in solution and at the mercury electrode surface
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SYSNO ASEP 0546616 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Voltammetric studies of selected porphyrin G-quadruplex ligands and their interaction with DNA in solution and at the mercury electrode surface Author(s) Dobrovodský, Daniel (BFU-R) ORCID
Daňhel, Aleš (BFU-R) RID, ORCID
Mothes-Martin, E. (FR)
Pratviel, G. (FR)
Mergny, Jean-Louis (BFU-R) ORCID, RID
Fojta, Miroslav (BFU-R) RID, ORCIDNumber of authors 6 Article number 139151 Source Title Electrochimica acta. - : Elsevier - ISSN 0013-4686
Roč. 394, OCT 20 2021 (2021)Number of pages 11 s. Publication form Online - E Language eng - English Country GB - United Kingdom Keywords cationic porphyrins ; metal-complexes ; binding ; metalloporphyrins ; selectivity Subject RIV CG - Electrochemistry OECD category Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) R&D Projects EF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 000703171900001 EID SCOPUS 85114124634 DOI 10.1016/j.electacta.2021.139151 Annotation With the increasing interest in DNA G-quadruplexes (G4) as important regulatory elements and prospective therapeutical targets, DNA structure-sensitive electrochemical methods can be advantageously used for studying of their structure or screening for interactions with G4 ligands developed as candidate drugs. Porphyrin derivatives are among the most prominent classes of G4 ligands. Interactions of porphyrins H 2TMPy2PP and Cu-TMPy2PP with oligodeoxynucleotides (ODNs) either forming or not forming G4 were investigated in this work. For this purpose, cyclic voltammetry at hanging mercury drop electrode (HMDE) complemented by UV-vis and CD spectroscopy was applied. Both porphyrins were found to interact strongly with G4 in solution by UV-vis titrations. However, Cu-TMPy2PP appears to interact also with double-stranded and single-stranded ODNs. Accordingly, cyclic voltammetry was able to distinguish between free and ODN-bound forms of Cu-TMPy2PP, but not between binding of the ligand to ODNs with or without G4 propensity. (C) 2021 Elsevier Ltd. All rights reserved. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2022 Electronic address https://www.sciencedirect.com/science/article/pii/S0013468621014419?via%3Dihub
Number of the records: 1