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Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals
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SYSNO ASEP 0546298 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals Author(s) Li, Y. (CN)
Leveau, A. (GB)
Zhao, Q. (US)
Martin, A. (DE)
Wegel, E. (GB)
Orme, A. (GB)
Karafiátová, Miroslava (UEB-Q) RID, ORCID
Vrána, Jan (UEB-Q) RID, ORCID
Steuernagel, B. (GB)
Joynson, R. (GB)
Louveau, T. (GB)
Stephenson, M. J. (GB)
Fan, D. (CN)
Zhou, C. (CN)
Tian, Q. (CN)
Polturak, G. (GB)
Casson, R. (GB)
Hill, L. (GB)
Moore, G. (GB)
Melton, R. (GB)
Hall, M. (GB)
Wulff, B. B. H. (GB)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Langdon, T.G. (GB)
Han, B. (US)
Osbourn, A. (GB)Number of authors 44 Article number 2563 Source Title Nature Communications. - : Nature Publishing Group
Roč. 12, č. 1 (2021)Number of pages 13 s. Language eng - English Country GB - United Kingdom Keywords In Situ Hybridization ; Fluorescence ; Repetitive Sequences OECD category Biochemistry and molecular biology R&D Projects EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000656449400002 EID SCOPUS 85105527354 DOI 10.1038/s41467-021-22920-8 Annotation Non-random gene organization in eukaryotes plays a significant role in genome evolution. Here, we investigate the origin of a biosynthetic gene cluster for production of defence compounds in oat—the avenacin cluster. We elucidate the structure and organisation of this 12-gene cluster, characterise the last two missing pathway steps, and reconstitute the entire pathway in tobacco by transient expression. We show that the cluster has formed de novo since the divergence of oats in a subtelomeric region of the genome that lacks homology with other grasses, and that gene order is approximately colinear with the biosynthetic pathway. We speculate that the positioning of the late pathway genes furthest away from the telomere may mitigate against a ‘self-poisoning’ scenario in which toxic intermediates accumulate as a result of telomeric gene deletions. Our investigations reveal a striking example of adaptive evolution underpinned by remarkable genome plasticity. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2022 Electronic address http://doi.org/10.1038/s41467-021-22920-8
Number of the records: 1