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Development of CE-C4d method for determination tropane alkaloids
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SYSNO ASEP 0546255 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Development of CE-C4d method for determination tropane alkaloids Author(s) Gołab, M. (PL)
Przybyłowska, M. (PL)
Kubáň, Petr (UIACH-O) ORCID
Itterheimová, Petra (UIACH-O) ORCID
Wózniakiewicz, M. (PL)Number of authors 5 Article number 5749 Source Title Molecules. - : MDPI
Roč. 26, č. 19 (2021)Number of pages 11 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords capillary electrophoresis ; tropane alkaloids ; conductivity detection Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry Method of publishing Open access Institutional support UIACH-O - RVO:68081715 UT WOS 000708212100001 EID SCOPUS 85115665380 DOI 10.3390/molecules26195749 Annotation A fast method for the determination of tropane alkaloids, using a portable CE instrument with a capacitively coupled contactless conductivity detector (CE-C4D) was developed and validated for determination of atropine and scopolamine in seeds from Solanaceae family plants. Sepa-ration was obtained within 5 min, using an optimized background electrolyte consisting of 0.5 M acetic acid with 0.25% (w/v) β-CD. The limit of detection and quantification was 0.5 µg/mL and 1.5 µg/mL, respectively, for both atropine and scopolamine. The developed method was validated with the following parameters—precision (CV): 1.07–2.08%, accuracy of the assay (recovery, RE): 101.0– 102.7% and matrix effect (ME): 92.99–94.23%. Moreover, the optimized CE-C4D method was applied to the analysis of plant extracts and pharmaceuticals, proving its applicability and accuracy. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2022 Electronic address https://www.mdpi.com/1420-3049/26/19/5749
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