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Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria
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SYSNO ASEP 0546092 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria Author(s) Obořilová, R. (CZ)
Šimečková, H. (CZ)
Pastucha, M. (CZ)
Klimovič, Š. (CZ)
Víšová, Ivana (FZU-D) ORCID
Přibyl, J. (CZ)
Vaisocherová-Lísalová, Hana (FZU-D) ORCID
Pantůček, R. (CZ)
Skládal, P. (CZ)
Mašlaňová, I. (CZ)
Farka, Z. (CZ)Number of authors 11 Source Title Nanoscale. - : Royal Society of Chemistry - ISSN 2040-3364
Roč. 13, č. 31 (2021), s. 13538-13549Number of pages 12 s. Language eng - English Country GB - United Kingdom Keywords atomic force microscopy ; AFM ; surface plasmon resonance ; SPR ; Staphylococcus aureus ; lysis ; lysostaphin ; bacteriophage ; phage therapy Subject RIV BO - Biophysics OECD category Biophysics Method of publishing Limited access Institutional support FZU-D - RVO:68378271 UT WOS 000680101900001 EID SCOPUS 85113132825 DOI 10.1039/d1nr02921e Annotation The growing incidence of multidrug-resistant bacterial strains presents a major challenge in modern medicine. Antibiotic resistance is often exhibited by Staphylococcus aureus, which causes severe infections in human and animal hosts and leads to significant economic losses. Antimicrobial agents with enzymatic activity (enzybiotics) and phage therapy represent promising and effective alternatives to classic antibiotics. However, new tools are needed to study phage–bacteria interactions and bacterial lysis with high resolution and in real-time. Here, we introduce a method for studying the lysis of S. aureus at the single-cell level in real-time using atomic force microscopy (AFM) in liquid. We demonstrate the ability of the method to monitor the effect of the enzyme lysostaphin on S. aureus and the lytic action of the Podoviridae phage P68. Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2022 Electronic address https://doi.org/10.1039/d1nr02921e
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