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Molecular organization of recombinant human-Arabidopsis chromosomes in hybrid cell lines
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SYSNO ASEP 0545987 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Molecular organization of recombinant human-Arabidopsis chromosomes in hybrid cell lines Author(s) Liu, Y. (AU)
Liaw, Y. M. (JP)
Teo, C. H. (MY)
Cápal, Petr (UEB-Q) RID, ORCID
Wada, N. (JP)
Fukui, K. (JP)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Ohmido, N. (JP)Number of authors 8 Article number 7160 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 11, č. 1 (2021)Number of pages 13 s. Language eng - English Country GB - United Kingdom Keywords Arabidopsis ; Fluorescence In Situ Hybridization ; hybrid cell line OECD category Biochemistry and molecular biology R&D Projects EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000636270100003 EID SCOPUS 85103682368 DOI 10.1038/s41598-021-86130-4 Annotation Although plants and animals are evolutionarily distant, the structure and function of their chromosomes are largely conserved. This allowed the establishment of a human-Arabidopsis hybrid cell line in which a neo-chromosome was formed by insertion of segments of Arabidopsis chromosomes into human chromosome 15. We used this unique system to investigate how the introgressed part of a plant genome was maintained in human genetic background. The analysis of the neo-chromosome in 60- and 300-day-old cell cultures by next-generation sequencing and molecular cytogenetics suggested its origin by fusion of DNA fragments of different sizes from Arabidopsis chromosomes 2, 3, 4, and 5, which were randomly intermingled rather than joined end-to-end. The neo-chromosome harbored Arabidopsis centromeric repeats and terminal human telomeres. Arabidopsis centromere wasn’t found to be functional. Most of the introgressed Arabidopsis DNA was eliminated during the culture, and the Arabidopsis genome in 300-day-old culture showed significant variation in copy number as compared with the copy number variation in the 60-day-old culture. Amplified Arabidopsis centromere DNA and satellite repeats were localized at particular loci and some fragments were inserted into various positions of human chromosome. Neo-chromosome reorganization and behavior in somatic cell hybrids between the plant and animal kingdoms are discussed. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2022 Electronic address http://doi.org/10.1038/s41598-021-86130-4
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