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Thermoresponsive properties of polyacrylamides in physiological solutions
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SYSNO ASEP 0545564 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Thermoresponsive properties of polyacrylamides in physiological solutions Author(s) Kolouchová, Kristýna (UMCH-V) ORCID, RID
Lobaz, Volodymyr (UMCH-V) RID, ORCID
Beneš, Hynek (UMCH-V) RID, ORCID
de la Rosa, V. R. (BE)
Babuka, David (UMCH-V)
Švec, Pavel (UMCH-V) RID, ORCID
Černoch, Peter (UMCH-V) RID, ORCID
Hrubý, Martin (UMCH-V) RID, ORCID
Hoogenboom, R. (BE)
Štěpánek, Petr (UMCH-V) RID, ORCID
Groborz, Ondřej (UMCH-V) ORCID, RIDSource Title Polymer Chemistry . - : Royal Society of Chemistry - ISSN 1759-9954
Roč. 12, č. 35 (2021), s. 5077-5084Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords lower critical solution temperature ; LCST ; cloud point temperature Subject RIV CD - Macromolecular Chemistry OECD category Polymer science R&D Projects GA19-01602S GA ČR - Czech Science Foundation (CSF) TO01000074 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) Method of publishing Open access Institutional support UMCH-V - RVO:61389013 UT WOS 000687745000001 EID SCOPUS 85115181597 DOI 10.1039/D1PY00843A Annotation Polymer solutions with a lower critical solution temperature (LCST) undergo reversible phase separation when heated above their cloud point temperature (TCP or CPT). As such, they have been proposed for a wide range of biomedical applications, from injectable drug depots to switchable coatings for cell adhesion. However, in systematic studies, the TCP of these thermoresponsive polymers has been mostly measured in non-physiological solutions, thereby hindering the development of their medicinal applications. Here, we analysed the thermoresponsive properties of four acrylamide-based polymers with LCST, namely poly[(N-2,2-difluoroethyl)acrylamide] (pDFEA), poly[(N-isopropyl)acrylamide] (pNIPAM), poly[(N,N-diethyl)acrylamide] (pDEA), and poly[(N-acryloyl)pyrrolidine] (pAP). As shown by turbidimetry, their TCP in phosphate saline buffer (PBS) and foetal bovine serum (FBS) were consistently lower than those reported in the literature, typically assessed in pure water, even when using the same setup. In addition, these physiological solutions affected the variation of TCP as a function of polymer concentration (1.25 to 10.0 mg mL−1) and molar mass (20 to 50 kg mol−1). As shown by isothermal calorimetry, interactions between proteins in FBS and polymer aggregates were predominantly exothermic, which indicates that protein–polymer complexes are formed through enthalpically driven processes. In conclusion, the TCP of thermoresponsive polymers strongly depends on solvent composition and therefore should be measured under physiological conditions for future medicinal applications.
Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2022 Electronic address https://pubs.rsc.org/en/content/articlelanding/2021/PY/D1PY00843A
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