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Lipid Nanoparticles for Broad-Spectrum Nucleic Acid Delivery
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SYSNO ASEP 0545469 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Lipid Nanoparticles for Broad-Spectrum Nucleic Acid Delivery Author(s) Hejdánková, Zuzana (UOCHB-X) ORCID
Vaněk, Václav (UOCHB-X) RID, ORCID
Sedlák, František (UOCHB-X) RID, ORCID
Procházka, Jan (UMG-J) ORCID
Diederichs, Audrey (UOCHB-X)
Kereiche, Sami (UOCHB-X) ORCID
Novotná, Barbora (UOCHB-X) ORCID
Buděšínský, Miloš (UOCHB-X) RID, ORCID
Birkuš, Gabriel (UOCHB-X) ORCID
Grantz Šašková, Klára (UOCHB-X) RID, ORCID
Cígler, Petr (UOCHB-X) RID, ORCIDArticle number 2101391 Source Title Advanced Functional Materials - ISSN 1616-301X
Roč. 31, č. 47 (2021)Number of pages 9 s. Language eng - English Country DE - Germany Keywords cyclic dinucleotides ; DNA ; drug delivery ; lipid nanoparticles ; RNA OECD category Biochemistry and molecular biology R&D Projects EF16_019/0000729 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018126 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001789 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF18_046/0015861 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED2.1.00/19.0395 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UOCHB-X - RVO:61388963 ; UMG-J - RVO:68378050 UT WOS 000688518800001 EID SCOPUS 85113371724 DOI https://doi.org/10.1002/adfm.202101391 Annotation Lipid nanoparticles (LNPs) are the most advanced nonviral modality for nucleic acid (NA) delivery, and have recently gained enormous attention in the fields of RNA therapeutics and vaccine development. Here, ionizable adamantane-based lipidoids named XMaNs, which circumvent the usual need for laborious optimization of LNP components for highly diverse types of NAs, are described. The non-toxic XMaN6 lipidoid is highly versatile in entrapment and delivery of siRNA, mRNA, plasmid DNA, and a cyclic dinucleotide. XMaN6-based LNPs efficiently deliver: 1) siRNA into human primary hepatocytes and cell lines that are hard-to-transfect, 2) mRNA into mouse liver, 3) plasmid DNA, 4) 2′,3′-cGAMP into cells and activated the cGAS-STING pathway three orders of magnitude more efficiently than 2′,3′-cGAMP alone. To our knowledge, such universality in delivering different NA types has not been previously described and can accelerate translation of LNPs into the clinic. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2022 Electronic address https://doi.org/10.1002/adfm.202101391
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