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Progress in forensic bone DNA analysis: Lessons learned from ancient DNA
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SYSNO ASEP 0545309 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Progress in forensic bone DNA analysis: Lessons learned from ancient DNA Author(s) Hofreiter, M. (DE)
Šneberger, Jiří (UJF-V) ORCID, RID, SAI
Pospíšek, M. (CZ)
Vaněk, D. (CZ)Number of authors 4 Article number 102538 Source Title Forensic Science International-Genetics. - : Elsevier - ISSN 1872-4973
Roč. 54, SEP (2021)Number of pages 15 s. Publication form Print - P Language eng - English Country IE - Ireland Keywords Ancient DNA ; forensic ; inhibitors ; degraded DNA Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) Method of publishing Limited access Institutional support UJF-V - RVO:61389005 UT WOS 000691184500004 EID SCOPUS 85110139606 DOI 10.1016/j.fsigen.2021.102538 Annotation Research on ancient and forensic DNA is related in many ways, and the two fields must deal with similar ob-stacles. Therefore, communication between these two communities has the potential to improve results in both research fields. Here, we present the insights gained in the ancient DNA community with regard to analyzing DNA from aged skeletal material and the potential use of the developed protocols in forensic work. We discuss the various steps, from choosing samples for DNA extraction to deciding between classical PCR amplification and massively parallel sequencing approaches. Based on the progress made in ancient DNA analyses combined with the requirements of forensic work, we suggest that there is substantial potential for incorporating ancient DNA approaches into forensic protocols, a process that has already begun to a considerable extent. However, taking full advantage of the experiences gained from ancient DNA work will require comparative studies by the forensic DNA community to tailor the methods developed for ancient samples to the specific needs of forensic studies and case work. If successful, in our view, the benefits for both communities would be considerable. Workplace Nuclear Physics Institute Contact Markéta Sommerová, sommerova@ujf.cas.cz, Tel.: 266 173 228 Year of Publishing 2022 Electronic address https://doi.org/10.1016/j.fsigen.2021.102538
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