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DDX21 is a p38-MAPK-sensitive nucleolar protein necessary for mouse preimplantation embryo development and cell-fate specification
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SYSNO ASEP 0545150 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title DDX21 is a p38-MAPK-sensitive nucleolar protein necessary for mouse preimplantation embryo development and cell-fate specification Author(s) Bora, P. (CZ)
Gahurová, Lenka (UZFG-Y) ORCID
Hauserová, A. (CZ)
Stiborová, M. (CZ)
Collier, R. (CZ)
Potěšil, D. (CZ)
Zdráhal, Z. (CZ)
Bruce, A. W. (CZ)Article number 210092 Source Title Open Biology. - : Royal Society Publishing
Roč. 11, č. 7 (2021)Number of pages 15 s. Publication form Online - E Language eng - English Country GB - United Kingdom Keywords DDX21 ; p38-MAPK ; preimplantation embryo development Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology Method of publishing Open access Institutional support UZFG-Y - RVO:67985904 UT WOS 000674669000001 EID SCOPUS 85111561222 DOI 10.1098/rsob.210092 Annotation Successful navigation of the mouse preimplantation stages of development, during which three distinct blastocyst lineages are derived, represents a prerequisite for continued development. We previously identified a role for p38-mitogen-activated kinases (p38-MAPK) regulating blastocyst inner cell mass (ICM) cell fate, specifically primitive endoderm (PrE) differentiation, that is intimately linked to rRNA precursor processing, polysome formation and protein translation regulation. Here, we develop this work by assaying the role of DEAD-box RNA helicase 21 (DDX21), a known regulator of rRNA processing, in the context of p38-MAPK regulation of preimplantation mouse embryo development. We show nuclear DDX21 protein is robustly expressed from the 16-cell stage, becoming exclusively nucleolar during blastocyst maturation, a localization dependent on active p38-MAPK. siRNA-mediated clonal Ddx21 knockdown within developing embryos is associated with profound cell-autonomous and non-autonomous proliferation defects and reduced blastocyst volume, by the equivalent peri-implantation blastocyst stage. Moreover, ICM residing Ddx21 knockdown clones express the EPI marker NANOG but rarely express the PrE differentiation marker GATA4. These data contribute further significance to the emerging importance of lineage-specific translation regulation, as identified for p38-MAPK, during mouse preimplantation development. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2022 Electronic address https://royalsocietypublishing.org/doi/10.1098/rsob.210092
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