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Human galectin-3: Molecular switch of gene expression in dermal fibroblasts in vitro and of skin collagen organization in open wounds and tensile strength in incisions in vivo
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SYSNO ASEP 0544840 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Human galectin-3: Molecular switch of gene expression in dermal fibroblasts in vitro and of skin collagen organization in open wounds and tensile strength in incisions in vivo Author(s) Gal, P. (SK)
Vasilenko, T. (SK)
Kovac, I. (SK)
Coma, M. (SK)
Jakubco, J. (SK)
Jakubcová, M. (CZ)
Perzelova, V. (SK)
Urban, L. (SK)
Kolář, Michal (UMG-J) RID, ORCID
Sabol, F. (SK)
Luczy, J. (SK)
Novotný, M. (SK)
Majernik, J. (SK)
Gabius, H. J. (DE)
Smetana, K. (CZ)Number of authors 15 Article number 99 Source Title Molecular Medicine Reports. - : Spandidos Publications - ISSN 1791-2997
Roč. 23, č. 2 (2021)Number of pages 11 s. Publication form Online - E Language eng - English Country GR - Greece Keywords extracellular matrix ; fibroblast ; keratinocyte ; lectin ; regeneration Subject RIV EB - Genetics ; Molecular Biology OECD category Oncology Method of publishing Open access Institutional support UMG-J - RVO:68378050 UT WOS 000601339700001 DOI 10.3892/mmr.2020.11738 Annotation Understanding the molecular and cellular processes in skin wound healing can pave the way for devising innovative concepts by turning the identified natural effectors into therapeutic tools. Based on the concept of broad-scale engagement of members of the family of galactoside-binding lectins (galectins) in pathophysiological processes, such as cancer or tissue repair/regeneration, the present study investigated the potential of galectins-1 (Gal-1) and3 (Gal-3) in wound healing. Human dermal fibroblasts, which are key cells involved in skin wound healing, responded to galectin exposure (Gal-1 at 300 or Gal-3 at 600 ng/ml) with selective changes in gene expression among a panel of 84 wound-healing-related genes, as well as remodeling of the extracellular matrix. In the case of Gal-3, positive expression of Ki67 and cell number increased when using a decellularized matrix produced by Gal-3-treated fibroblasts as substrate for culture of interfollicular keratinocytes. In vivo wounds were topically treated with 20 ng/ml Gal-1 or3, and collagen score was found to be elevated in excisional wound repair in rats treated with Gal-3. The tensile strength measured in incisions was significantly increased from 79.5 +/- 17.5 g/mm(2) in controls to 103.1 +/- 21.4 g/mm(2) after 21 days of healing. These data warrant further testing mixtures of galectins and other types of compounds, for example a combination of galectins and TGF-beta 1. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2022 Electronic address https://www.spandidos-publications.com/10.3892/mmr.2020.11738
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