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Polarization-resolved second-harmonic generation imaging through a multimode fiber

SYSNO ASEP	0544708
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Polarization-resolved second-harmonic generation imaging through a multimode fiber
Author(s)	Cifuentes, Angel S. (UPT-D)_{RID, SAI, ORCID} Pikálek, Tomáš (UPT-D)_{RID, ORCID, SAI} Ondráčková, Petra (UPT-D) Amezcua-Correa, R. (US) Antonio-Lopez, J. E. (US) Čižmár, Tomáš (UPT-D)_{RID, ORCID, SAI} Traegaardh, Johanna (UPT-D)_{RID, ORCID, SAI}
Number of authors	7
Source Title	Optica. - : Optical Society of America - ISSN 2334-2536 Roč. 8, č. 8 (2021), s. 1065-1074
Number of pages	10 s.
Publication form	Online - E
Language	eng - English
Country	US - United States
Keywords	second harmonic generation ; multimode fiber ; bioimaging ; polarization control
Subject RIV	BH - Optics, Masers, Lasers
OECD category	Optics (including laser optics and quantum optics)
R&D Projects	EF15_003/0000476 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	UPT-D - RVO:68081731
UT WOS	000687747700005
EID SCOPUS	85111981587
DOI	10.1364/OPTICA.430295
Annotation	Multimode fiber-based endoscopes have recently emerged as a tool for minimally invasive endoscopy in tissue, at depths well beyond the reach of multiphoton imaging. Here, we demonstrate label-free second-harmonic generation (SHG) microscopy through such a fiber endoscope. We simultaneously fully control the excitation polarization state and the spatial distribution of the light at the fiber tip, and we use this to implement polarization-resolved SHG imaging, which allows imaging and identification of structural proteins such as collagen and myosin.We image mouse tail tendon and heart tissue, employing the endoscope at depths up to 1 mm, demonstrating that we can differentiate these structural proteins. This method has the potential for enabling instant and in situ diagnosis of tumors and fibrotic conditions insensitive tissue with minimal damage.
Workplace	Institute of Scientific Instruments
Contact	Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
Year of Publishing	2022
Electronic address	https://www.osapublishing.org/optica/fulltext.cfm?uri=optica-8-8-1065&id=453930

Number of the records: 1