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Physical Chemistry and Chemical Physics Editor’s Pick 2021

SYSNO ASEP	0544683
Document Type	M - Monograph Chapter
R&D Document Type	The record was not marked in the RIV
Title	Monodisperse core-shell NaYF4:Yb3+/Er3+@NaYF4:Nd3+-PEG GGGRGDSGGGY-NH2 nanoparticles excitable at 808 and 980 nm: design, surface engineering, and application in life sciences
Author(s)	Kostiv, Uliana (UMCH-V)_RID Engstová, Hana (FGU-C)_{RID, ORCID} Krajnik, B. (PL) Šlouf, Miroslav (UMCH-V)_{RID, ORCID} Proks, Vladimír (UMCH-V)_{RID, ORCID} Podhorodecki, A. (PL) Ježek, Petr (FGU-C)_{RID, ORCID} Horák, Daniel (UMCH-V)_{RID, ORCID}
Source Title	Physical Chemistry and Chemical Physics Editor’s Pick 2021. - Lausanne : Frontiers Media SA, 2021 / Biczysko Malgorzata - ISSN 1664-8714 - ISBN 978-2-88971-110-9
Pages	s. 104-118
Number of pages	15 s.
Number of pages	193
Publication form	Online - E
Language	eng - English
Country	CH - Switzerland
Keywords	upconversion nanoparticles ; core-shell ; 808 nm excitation
Subject RIV	CD - Macromolecular Chemistry
OECD category	Polymer science
Subject RIV - cooperation	Institute of Physiology - Cell Biology
R&D Projects	GA19-00676S GA ČR - Czech Science Foundation (CSF)
	TE01020118 GA TA ČR - Technology Agency of the Czech Republic (TA ČR)
	LO1507 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA18-05510S GA ČR - Czech Science Foundation (CSF)
	8JPL19006 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UMCH-V - RVO:61389013 ; FGU-C - RVO:67985823
DOI	10.3389/978-2-88971-110-9
Annotation	Lanthanide-doped upconversion nanoparticles (UCNPs) have a unique capability of upconverting near-infrared (NIR) excitation into ultraviolet, visible, and NIR emission. Conventional UCNPs composed of NaYF4:Yb3+/Er3+(Tm3+) are excited by NIR light at 980 nm, where undesirable absorption by water can cause overheating or damage of living tissues and reduce nanoparticle luminescence. Incorporation of Nd3+ ions into the UCNP lattice shifts the excitation wavelength to 808 nm, where absorption of water is minimal. Herein, core-shell NaYF4:Yb3+/Er3+@NaYF4:Nd3+ nanoparticles, which are doubly doped by sensitizers (Yb3+ and Nd3+) and an activator (Er3+) in the host NaYF4 matrix, were synthesized by high-temperature coprecipitation of lanthanide chlorides in the presence of oleic acid as a stabilizer. Uniform core (24 nm) and core-shell particles with tunable shell thickness (~0.5–4 nm) were thoroughly characterized by transmission electron microscopy (TEM), energy-dispersive analysis, selected area electron diffraction, and photoluminescence emission spectra at 808 and 980 nm excitation. To ensure dispersibility of the particles in biologically relevant media, they were coated by in-house synthesized poly(ethylene glycol) (PEG)-neridronate terminated with an alkyne (Alk). The stability of the NaYF4:Yb3+/Er3+@NaYF4:Nd3+-PEG-Alk nanoparticles in water or 0.01 M PBS and the presence of PEG on the surface were determined by dynamic light scattering, ζ-potential measurements, thermogravimetric analysis, and FTIR spectroscopy. Finally, the adhesive azidopentanoyl-modified GGGRGDSGGGY-NH2 (RGDS) peptide was immobilized on the NaYF4:Yb3+/Er3+@NaYF4:Nd3+-PEG-Alk particles via Cu(I)-catalyzed azide-alkyne cycloaddition. The toxicity of the unmodified core-shell NaYF4:Yb3+/Er3+@NaYF4:Nd3+, NaYF4:Yb3+/Er3+@NaYF4:Nd3+-PEG-Alk, and NaYF4:Yb3+/Er3+@NaYF4:Nd3+-PEG-RGDS nanoparticles on both Hep-G2 and HeLa cells was determined, confirming no adverse effect on their survival and proliferation. The interaction of the nanoparticles with Hep-G2 cells was monitored by confocal microscopy at both 808 and 980 nm excitation. The NaYF4:Yb3+/Er3+@NaYF4:Nd3+-PEG-RGDS nanoparticles were localized on the cell membranes due to specific binding of the RGDS peptide to integrins, in contrast to the NaYF4:Yb3+/Er3+@NaYF4:Nd3+-PEG-Alk particles, which were not engulfed by the cells. The NaYF4:Yb3+/Er3+@NaYF4:Nd3+-PEG-RGDS nanoparticles thus appear to be promising as a new non-invasive probe for specific bioimaging of cells and tissues. This development makes the nanoparticles useful for diagnostic and/or, after immobilization of a bioactive compound, even theranostic applications in the treatment of various fatal diseases.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2022
Electronic address	https://www.frontiersin.org/research-topics/21896/physical-chemistry-and-chemical-physics-editors-pick-2021

Number of the records: 1