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Anhydrobiosis in yeast: role of cortical endoplasmic reticulum protein Ist2 in Saccharomyces cerevisiae cells during dehydration and subsequent rehydration
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SYSNO ASEP 0543905 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Anhydrobiosis in yeast: role of cortical endoplasmic reticulum protein Ist2 in Saccharomyces cerevisiae cells during dehydration and subsequent rehydration Author(s) Dauss, E. (LT)
Papoušková, Klára (FGU-C) RID, ORCID
Sychrová, Hana (FGU-C) RID, ORCID
Rapoport, A. (LT)Source Title Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology. - : Springer - ISSN 0003-6072
Roč. 114, č. 7 (2021), s. 1069-1077Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords anhydrobiosis ; dehydration-rehydration ; yeast ; endoplasmic reticulum ; endoplasmic reticulum protein Ist2 Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects LTC20006 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support FGU-C - RVO:67985823 UT WOS 000639508200002 EID SCOPUS 85104409085 DOI 10.1007/s10482-021-01578-8 Annotation Two Saccharomyces cerevisiae strains, BY4741 and BY4741-derived strain lacking the IST2 gene (ist2 Delta), were used to characterise the possible role of cortical endoplasmic reticulum (ER) protein Ist2 upon cell dehydration and subsequent rehydration. For the first time, we show that not only protein components of the plasma membrane (PM), but also at least one ER membrane protein (Ist2) play an important role in the maintenance of the viability of yeast cells during dehydration and subsequent rehydration. The low viability of the mutant strain ist2 increment upon dehydration-rehydration stress was related to the lack of Ist2 protein in the ER. We revealed that the PM of ist2 increment strain is not able to completely restore its molecular organisation during reactivation from the state of anhydrobiosis. As the result, the permeability of the PM remains high regardless of the type of reactivation (rapid or gradual rehydration). We conclude that ER protein Ist2 plays an important role in ensuring the stability of molecular organisation and functionality of the PM during dehydration-rehydration stress. These results indicate an important role of ER-PM interactions during cells transition into the state of anhydrobiosis and the subsequent restoration of their physiological activities. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2022 Electronic address https://doi.org/10.1007/s10482-021-01578-8
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