Interaction between Galectin-3 and Integrins Mediates Cell-Matrix Adhesion in Endothelial Cells and Mesenchymal Stem Cells

Sedlář, Antonín; Trávníčková, Martina; Bojarová, Pavla; Vlachová, Miluše; Slámová, Kristýna; Křen, Vladimír; Bačáková, Lucie

doi:https://dx.doi.org/10.3390/ijms22105144

Number of the records: 1

Interaction between Galectin-3 and Integrins Mediates Cell-Matrix Adhesion in Endothelial Cells and Mesenchymal Stem Cells

1.

SYSNO ASEP	0543839
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Interaction between Galectin-3 and Integrins Mediates Cell-Matrix Adhesion in Endothelial Cells and Mesenchymal Stem Cells
Author(s)	Sedlář, Antonín (FGU-C)_ORCID Trávníčková, Martina (FGU-C)_{RID, ORCID, SAI} Bojarová, Pavla (MBU-M)_ORCID Vlachová, Miluše (MBU-M)_ORCID Slámová, Kristýna (MBU-M)_{RID, ORCID} Křen, Vladimír (MBU-M)_{RID, ORCID} Bačáková, Lucie (FGU-C)_{RID, ORCID}
Article number	5144
Source Title	International Journal of Molecular Sciences. - : MDPI Roč. 22, č. 10 (2021)
Number of pages	26 s.
Language	eng - English
Country	CH - Switzerland
Keywords	galectin ; HUVEC ; ADSC ; integrin ; carbohydrate
Subject RIV	EI - Biotechnology ; Bionics
OECD category	Biomaterials (as related to medical implants, devices, sensors)
Subject RIV - cooperation	Institute of Microbiology - Biotechnology ; Bionics
R&D Projects	LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EF18_046/0016045 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA18-01163S GA ČR - Czech Science Foundation (CSF)
	LTC18041 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LTC18038 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823 ; MBU-M - RVO:61388971
UT WOS	000662006700001
EID SCOPUS	85105699642
DOI	10.3390/ijms22105144
Annotation	Galectin-3 (Gal-3) is a beta-galactoside-binding protein that influences various cell functions, including cell adhesion. We focused on the role of Gal-3 as an extracellular ligand mediating cell-matrix adhesion. We used human adipose tissue-derived stem cells and human umbilical vein endothelial cells that are promising for vascular tissue engineering. We found that these cells naturally contained Gal-3 on their surface and inside the cells. Moreover, they were able to associate with exogenous Gal-3 added to the culture medium. This association was reduced with a beta-galactoside LacdiNAc (GalNAc beta 1,4GlcNAc), a selective ligand of Gal-3, which binds to the carbohydrate recognition domain (CRD) in the Gal-3 molecule. This ligand was also able to detach Gal-3 newly associated with cells but not Gal-3 naturally present on cells. In addition, Gal-3 preadsorbed on plastic surfaces acted as an adhesion ligand for both cell types, and the cell adhesion was resistant to blocking with LacdiNAc. This result suggests that the adhesion was mediated by a binding site different from the CRD. The blocking of integrin adhesion receptors on cells with specific antibodies revealed that the cell adhesion to the preadsorbed Gal-3 was mediated, at least partially, by beta 1 and alpha V integrins-namely alpha 5 beta 1, alpha V beta 3, and alpha V beta 1 integrins.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2022
Electronic address	https://www.mdpi.com/1422-0067/22/10/5144

Number of the records: 1