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Covalent cationic copolymer coatings allowing tunable electroosmotic flow for optimization of capillary electrophoretic separations

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    SYSNO ASEP0543809
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleCovalent cationic copolymer coatings allowing tunable electroosmotic flow for optimization of capillary electrophoretic separations
    Author(s) Konášová, Renáta (UOCHB-X) ORCID
    Butnariu, Maria (UOCHB-X) ORCID
    Šolínová, Veronika (UOCHB-X) RID, ORCID
    Kašička, Václav (UOCHB-X) RID, ORCID
    Koval, Dušan (UOCHB-X) RID, ORCID
    Article number338789
    Source TitleAnalytica Chimica Acta. - : Elsevier - ISSN 0003-2670
    Roč. 1178, Sep 15 (2021)
    Number of pages8 s.
    Languageeng - English
    CountryNL - Netherlands
    Keywordselectroosmotic flow ; capillary electrophoresis ; mass spectrometry ; permanent polymeric coating ; sorption suppression
    OECD categoryAnalytical chemistry
    R&D ProjectsGA20-03899S GA ČR - Czech Science Foundation (CSF)
    Method of publishingLimited access
    Institutional supportUOCHB-X - RVO:61388963
    UT WOS000672564300008
    EID SCOPUS85109129026
    DOI10.1016/j.aca.2021.338789
    AnnotationElectroosmotic flow (EOF) plays a pivotal role in optimization of capillary electrophoresis (CE) separations of (bio)molecules and (bio)particles. EOF velocity is directly related to analysis time, peak resolution and separation efficiency. Here, we report a concept of charged polymer coatings of the inner fused silica capillary wall, which allows anodic EOF with mobility ranging from 0 to ∼(30–40) × 10−9 m2V−1s−1. The capillary wall is modified by covalently bound cationic copolymer poly(acrylamide-co-(3-acrylamidopropyl)trimethylammonium chloride) (PAMAPTAC) containing variable ratio of the charged monomer in the 0–60 mol. % interval. The EOF mobility showed minor variability with composition of background electrolyte (BGE) and pH in the 2–10 interval. The coatings were evaluated by CE-UV and nanospray CE-MS in the counter-EOF arrangement for a series of basic drug molecules in acetic acid based acidic BGE. Tunable EOF velocity was demonstrated as a useful tool for optimization of peak resolution, separation efficiency and migration time of analytes. Electrostatic repulsion of positively charged capillary surface was shown as beneficial for suppression of analyte adsorption, notably for hydrophobic cationic analytes.
    WorkplaceInstitute of Organic Chemistry and Biochemistry
    Contactasep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
    Year of Publishing2022
    Electronic addresshttps://doi.org/10.1016/j.aca.2021.338789
Number of the records: 1