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Covalent cationic copolymer coatings allowing tunable electroosmotic flow for optimization of capillary electrophoretic separations
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SYSNO ASEP 0543809 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Covalent cationic copolymer coatings allowing tunable electroosmotic flow for optimization of capillary electrophoretic separations Author(s) Konášová, Renáta (UOCHB-X) ORCID
Butnariu, Maria (UOCHB-X) ORCID
Šolínová, Veronika (UOCHB-X) RID, ORCID
Kašička, Václav (UOCHB-X) RID, ORCID
Koval, Dušan (UOCHB-X) RID, ORCIDArticle number 338789 Source Title Analytica Chimica Acta. - : Elsevier - ISSN 0003-2670
Roč. 1178, Sep 15 (2021)Number of pages 8 s. Language eng - English Country NL - Netherlands Keywords electroosmotic flow ; capillary electrophoresis ; mass spectrometry ; permanent polymeric coating ; sorption suppression OECD category Analytical chemistry R&D Projects GA20-03899S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support UOCHB-X - RVO:61388963 UT WOS 000672564300008 EID SCOPUS 85109129026 DOI 10.1016/j.aca.2021.338789 Annotation Electroosmotic flow (EOF) plays a pivotal role in optimization of capillary electrophoresis (CE) separations of (bio)molecules and (bio)particles. EOF velocity is directly related to analysis time, peak resolution and separation efficiency. Here, we report a concept of charged polymer coatings of the inner fused silica capillary wall, which allows anodic EOF with mobility ranging from 0 to ∼(30–40) × 10−9 m2V−1s−1. The capillary wall is modified by covalently bound cationic copolymer poly(acrylamide-co-(3-acrylamidopropyl)trimethylammonium chloride) (PAMAPTAC) containing variable ratio of the charged monomer in the 0–60 mol. % interval. The EOF mobility showed minor variability with composition of background electrolyte (BGE) and pH in the 2–10 interval. The coatings were evaluated by CE-UV and nanospray CE-MS in the counter-EOF arrangement for a series of basic drug molecules in acetic acid based acidic BGE. Tunable EOF velocity was demonstrated as a useful tool for optimization of peak resolution, separation efficiency and migration time of analytes. Electrostatic repulsion of positively charged capillary surface was shown as beneficial for suppression of analyte adsorption, notably for hydrophobic cationic analytes. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2022 Electronic address https://doi.org/10.1016/j.aca.2021.338789
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