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Raman Microspectroscopic Analysis of Selenium Bioaccumulation by Green Alga Chlorella vulgaris
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SYSNO ASEP 0543532 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Raman Microspectroscopic Analysis of Selenium Bioaccumulation by Green Alga Chlorella vulgaris Author(s) Kizovský, Martin (UPT-D)
Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Mylenko, Mykola (MBU-M) ORCID
Hrouzek, Pavel (MBU-M) ORCID
Kuta, J. (CZ)
Skoupý, Radim (UPT-D) RID, ORCID, SAI
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Hrubanová, Kamila (UPT-D) RID, SAI, ORCID
Adamczyk, O. (PL)
Ježek, Jan (UPT-D) RID, ORCID, SAI
Bernatová, Silvie (UPT-D) RID, SAI, ORCID
Klementová, Tereza (UPT-D) ORCID, SAI
Gjevik, Alžběta (UPT-D)
Šiler, Martin (UPT-D) RID, ORCID, SAI
Samek, Ota (UPT-D) RID, ORCID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCIDNumber of authors 16 Article number 115 Source Title Biosensors. - : MDPI
Roč. 11, č. 4 (2021)Number of pages 13 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords selenium ; algae ; Raman spectroscopy ; EDX ; ICP-MS ; bioaccumulation ; Chlorella vulgaris Subject RIV BH - Optics, Masers, Lasers OECD category Electrical and electronic engineering Subject RIV - cooperation Institute of Microbiology - Microbiology, Virology R&D Projects LO1212 GA MŠk - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠk - Ministry of Education, Youth and Sports (MEYS) LO1416 GA MŠk - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UPT-D - RVO:68081731 ; MBU-M - RVO:61388971 UT WOS 000642778300001 EID SCOPUS 85105169745 DOI 10.3390/bios11040115 Annotation Selenium (Se) is an element with many commercial applications as well as an essential micronutrient. Dietary Se has antioxidant properties and it is known to play a role in cancer prevention. However, the general population often suffers from Se deficiency. Green algae, such as Chlorella vulgaris, cultivated in Se-enriched environment may be used as a food supplement to provide adequate levels of Se. We used Raman microspectroscopy (RS) for fast, reliable, and non-destructive measurement of Se concentration in living algal cells. We employed inductively coupled plasma-mass spectrometry as a reference method to RS and we found a substantial correlation between the Raman signal intensity at 252 cm(-1) and total Se concentration in the studied cells. We used RS to assess the uptake of Se by living and inactivated algae and demonstrated the necessity of active cellular transport for Se accumulation. Additionally, we observed the intracellular Se being transformed into an insoluble elemental form, which we further supported by the energy-dispersive X-ray spectroscopy imaging. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2022 Electronic address https://www.mdpi.com/2079-6374/11/4/115
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