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Raman Microspectroscopic Analysis of Selenium Bioaccumulation by Green Alga Chlorella vulgaris

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    SYSNO ASEP0543532
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleRaman Microspectroscopic Analysis of Selenium Bioaccumulation by Green Alga Chlorella vulgaris
    Author(s) Kizovský, Martin (UPT-D)
    Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
    Mylenko, Mykola (MBU-M) ORCID
    Hrouzek, Pavel (MBU-M) ORCID
    Kuta, J. (CZ)
    Skoupý, Radim (UPT-D) RID, ORCID, SAI
    Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
    Hrubanová, Kamila (UPT-D) RID, SAI, ORCID
    Adamczyk, O. (PL)
    Ježek, Jan (UPT-D) RID, ORCID, SAI
    Bernatová, Silvie (UPT-D) RID, SAI, ORCID
    Klementová, Tereza (UPT-D) ORCID, SAI
    Gjevik, Alžběta (UPT-D)
    Šiler, Martin (UPT-D) RID, ORCID, SAI
    Samek, Ota (UPT-D) RID, ORCID, SAI
    Zemánek, Pavel (UPT-D) RID, SAI, ORCID
    Number of authors16
    Article number115
    Source TitleBiosensors. - : MDPI
    Roč. 11, č. 4 (2021)
    Number of pages13 s.
    Publication formOnline - E
    Languageeng - English
    CountryCH - Switzerland
    Keywordsselenium ; algae ; Raman spectroscopy ; EDX ; ICP-MS ; bioaccumulation ; Chlorella vulgaris
    Subject RIVBH - Optics, Masers, Lasers
    OECD categoryElectrical and electronic engineering
    Subject RIV - cooperationInstitute of Microbiology - Microbiology, Virology
    R&D ProjectsLO1212 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    ED0017/01/01 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    LO1416 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    Method of publishingOpen access
    Institutional supportUPT-D - RVO:68081731 ; MBU-M - RVO:61388971
    UT WOS000642778300001
    EID SCOPUS85105169745
    DOI10.3390/bios11040115
    AnnotationSelenium (Se) is an element with many commercial applications as well as an essential micronutrient. Dietary Se has antioxidant properties and it is known to play a role in cancer prevention. However, the general population often suffers from Se deficiency. Green algae, such as Chlorella vulgaris, cultivated in Se-enriched environment may be used as a food supplement to provide adequate levels of Se. We used Raman microspectroscopy (RS) for fast, reliable, and non-destructive measurement of Se concentration in living algal cells. We employed inductively coupled plasma-mass spectrometry as a reference method to RS and we found a substantial correlation between the Raman signal intensity at 252 cm(-1) and total Se concentration in the studied cells. We used RS to assess the uptake of Se by living and inactivated algae and demonstrated the necessity of active cellular transport for Se accumulation. Additionally, we observed the intracellular Se being transformed into an insoluble elemental form, which we further supported by the energy-dispersive X-ray spectroscopy imaging.
    WorkplaceInstitute of Scientific Instruments
    ContactMartina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
    Year of Publishing2022
    Electronic addresshttps://www.mdpi.com/2079-6374/11/4/115
Number of the records: 1