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Monitoring fitness and productivity in cyanobacteria batch cultures

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    SYSNO ASEP0543490
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleMonitoring fitness and productivity in cyanobacteria batch cultures
    Author(s) Zavřel, Tomáš (UEK-B) RID, SAI, ORCID
    Schoffman, H. (IL)
    Lukeš, Martin (MBU-M) ORCID
    Fedorko, Jan (UEK-B) SAI, ORCID
    Keren, N. (IL)
    Červený, Jan (UEK-B) RID, ORCID, SAI
    Number of authors6
    Article number102328
    Source TitleAlgal Research-Biomass Biofuels and Bioproducts - ISSN 2211-9264
    Roč. 56, JUN (2021)
    Number of pages15 s.
    Languageeng - English
    CountryNL - Netherlands
    Keywordssp pcc 6803 ; photosystem-ii ; microalgae cultivation ; dark respiration ; growth-rate ; light ; phytoplankton ; photosynthesis ; fluorescence ; algae ; Batch cultivation ; Cyanobacteria ; Excitation-emission spectra ; Fitness ; Metal uptake ; Photosynthesis
    Subject RIVEE - Microbiology, Virology
    OECD categoryMicrobiology
    Subject RIV - cooperationInstitute of Microbiology - Microbiology, Virology
    R&D ProjectsLO1415 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    GA18-24397S GA ČR - Czech Science Foundation (CSF)
    EF16_026/0008413 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    TN01000048 GA TA ČR - Technology Agency of the Czech Republic (TA ČR)
    Research InfrastructureCzeCOS III - 90123 - Ústav výzkumu globální změny AV ČR, v. v. i.
    Method of publishingLimited access
    Institutional supportUEK-B - RVO:86652079 ; MBU-M - RVO:61388971
    UT WOS000654306500008
    EID SCOPUS85108537722
    DOI10.1016/j.algal.2021.102328
    AnnotationCyanobacteria are key photosynthetic organisms in many aquatic ecosystems and hold great potential for sustainable green biotechnology. Growth of cyanobacteria in batch cultures is expected to be part of future biotechnological practices. However, the issue of correlating the dynamics of metabolic and photosynthetic parameters with the culture fitness during batch cultivation is still outstanding. In this paper we take advantage of a photobioreactor system to continuously track growth parameters of Synechocystis sp. PCC 6803, and to couple online culture monitoring with offline measurements of photosynthetic efficiency and biochemical and elemental cell composition under several light intensity and CO2 regimes. Light intensity determines the flux of energy into the photosynthetic system while CO2 concentrations determines the ability to capture this energy in chemical form. From this perspective, four distinct source-sink regimes were established and compared, which allowed us to reveal specific strategies to acclimate to both carbon and light limitation. As part of the measurements, room temperature excitation-emission spectra and elemental composition of Synechocystis cells were, for the first time, compared throughout the exponential and linear growth phases. In total, 39 parameters (out of 170 measured) were identified as highly correlating (R2 > 0.9) with growth rate or productivity under at least one tested cultivation condition, including concentrations and ratios of pigments or particular elements. For online fitness and productivity monitoring in cyanobacteria batch cultures, parameters such as photosynthesis and respiration rates and ratios, energy-dependent non-photochemical quenching (qE) or Zn and Mo concentration in the cultivation medium can be of interest.
    WorkplaceGlobal Change Research Institute
    ContactNikola Audesová, audesova.n@czechglobe.cz, Tel.: 511 192 268
    Year of Publishing2022
    Electronic addresshttps://www.sciencedirect.com/science/article/pii/S2211926421001478?via%3Dihub
Number of the records: 1