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Monitoring fitness and productivity in cyanobacteria batch cultures
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SYSNO ASEP 0543490 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Monitoring fitness and productivity in cyanobacteria batch cultures Author(s) Zavřel, Tomáš (UEK-B) RID, SAI, ORCID
Schoffman, H. (IL)
Lukeš, Martin (MBU-M) ORCID
Fedorko, Jan (UEK-B) SAI, ORCID
Keren, N. (IL)
Červený, Jan (UEK-B) RID, ORCID, SAINumber of authors 6 Article number 102328 Source Title Algal Research-Biomass Biofuels and Bioproducts. - : Elsevier - ISSN 2211-9264
Roč. 56, JUN (2021)Number of pages 15 s. Language eng - English Country NL - Netherlands Keywords sp pcc 6803 ; photosystem-ii ; microalgae cultivation ; dark respiration ; growth-rate ; light ; phytoplankton ; photosynthesis ; fluorescence ; algae ; Batch cultivation ; Cyanobacteria ; Excitation-emission spectra ; Fitness ; Metal uptake ; Photosynthesis Subject RIV EE - Microbiology, Virology OECD category Microbiology Subject RIV - cooperation Institute of Microbiology - Microbiology, Virology R&D Projects LO1415 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA18-24397S GA ČR - Czech Science Foundation (CSF) EF16_026/0008413 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) TN01000048 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) Research Infrastructure CzeCOS III - 90123 - Ústav výzkumu globální změny AV ČR, v. v. i. Method of publishing Limited access Institutional support UEK-B - RVO:86652079 ; MBU-M - RVO:61388971 UT WOS 000654306500008 EID SCOPUS 85108537722 DOI 10.1016/j.algal.2021.102328 Annotation Cyanobacteria are key photosynthetic organisms in many aquatic ecosystems and hold great potential for sustainable green biotechnology. Growth of cyanobacteria in batch cultures is expected to be part of future biotechnological practices. However, the issue of correlating the dynamics of metabolic and photosynthetic parameters with the culture fitness during batch cultivation is still outstanding. In this paper we take advantage of a photobioreactor system to continuously track growth parameters of Synechocystis sp. PCC 6803, and to couple online culture monitoring with offline measurements of photosynthetic efficiency and biochemical and elemental cell composition under several light intensity and CO2 regimes. Light intensity determines the flux of energy into the photosynthetic system while CO2 concentrations determines the ability to capture this energy in chemical form. From this perspective, four distinct source-sink regimes were established and compared, which allowed us to reveal specific strategies to acclimate to both carbon and light limitation. As part of the measurements, room temperature excitation-emission spectra and elemental composition of Synechocystis cells were, for the first time, compared throughout the exponential and linear growth phases. In total, 39 parameters (out of 170 measured) were identified as highly correlating (R2 > 0.9) with growth rate or productivity under at least one tested cultivation condition, including concentrations and ratios of pigments or particular elements. For online fitness and productivity monitoring in cyanobacteria batch cultures, parameters such as photosynthesis and respiration rates and ratios, energy-dependent non-photochemical quenching (qE) or Zn and Mo concentration in the cultivation medium can be of interest. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2022 Electronic address https://www.sciencedirect.com/science/article/pii/S2211926421001478?via%3Dihub
Number of the records: 1